姜辣素拮抗掌叶半夏毒针晶刺激性毒性的机制研究  

Antagonism mechanism of gingerols against inflammatory effect of toxic raphides from Pinella pedatisecta

作　　者：金羊平 吴皓[1,2,3] 郁红礼[1,2,3] 潘耀宗[1] 陈叶青[1] 王奎龙[1] 张程超 王卫[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]江苏省中药炮制重点实验室,江苏南京210046 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210046

出　　处：《中国中药杂志》2016年第6期1087-1092,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173549);教育部博士点新教师基金项目(20113237120010);江苏高校优势学科建设工程项目

摘　　要：为探索姜辣素拮抗掌叶半夏毒针晶刺激性毒性的机制,该研究采用掌叶半夏毒针晶诱导的小鼠腹腔炎症模型,观察姜辣素对小鼠腹腔炎症渗出液中炎症介质PGE2的影响;采用大鼠腹腔巨噬细胞体外培养模型,以上清液中TNF-α和IL-1β为指标,研究姜辣素拮抗掌叶半夏毒针晶的致炎作用;采用扫描电镜法观察姜辣素拮抗掌叶半夏毒针晶刺激后巨噬细胞表面形态的改变;采用巨噬细胞-中性粒细胞共培养模型,考察姜辣素拮抗掌叶半夏毒针晶对中性粒细胞迁移的影响。结果显示,姜辣素可以显著抑制掌叶半夏毒针晶所致的小鼠腹腔渗出液中PGE2的生成;姜辣素可以显著抑制掌叶半夏毒针晶诱导巨噬细胞释放炎症因子,并具有一定的量效关系;扫描电镜显示,姜辣素可以显著抑制巨噬细胞吞噬掌叶半夏毒针晶及细胞膜破损,并能显著抑制掌叶半夏毒针晶诱导的中性粒细胞迁移。姜辣素拮抗掌叶半夏毒针晶刺激性毒性的机制可能是抑制了包括巨噬细胞激活、炎症因子释放、中性粒细胞迁移聚集的致炎毒性。This study was to investigate the mechanism of gingerols antagonizing the inflammatory effect of toxic raphides from Pinella pedatisecta. Mice peritonitis models induced by toxic raphides from P. pedatisecta were applied to observe the effect of gingerols on inflammatory mediators PGE2 in the exudates of abdominal inflammation in mice; rats peritoneal macrophage in vitro culture models were adopted to study the anti-inflammatory effects of gingerol against toxic raphides,with TNF-α and IL-1β in supernatant as indexes.Scanning electron microscopy was used to observe the changes in surface morphology of macrophages treated by raphides and gingerols.Macrophages-neutrophils co-cultured models were used to study the antagonism of gingerols against the effect of toxic raphides＇ stimulation on neutrophils migration. Results showed that gingerols could significantly inhibit the production of PGE2 in the exudates of abdominal inflammation induced by toxic raphides from P. pedatisecta in mice. Gingerols could significantly inhibit the toxic raphides from P. pedatisecta to induce the release of inflammatory factors,with certain dose dependence. Scanning electron microscopy showed that gingerols could significantly inhibit phagocytosis of macrophages,cytomembrane injury,and neutrophils migration induced by toxic raphides from P. pedatisecta. The results showed that the antagonism mechanism of gingerols against the toxic raphides from P. pedatisecta may be associated with inhibiting the pro-inflammatory toxicity including macrophage activation,inflammatory factors release,and neutrophils migration.

关 键 词：姜辣素 掌叶半夏 巨噬细胞 炎症因子 扫描电镜 中性粒细胞趋化 

分 类 号：R285.5[医药卫生—中药学]