三相法从苦杏仁中分离制备β-葡萄糖苷酶  

Preparation of β-Glucosidase from Bitter Almond by Three-phase Partitioning

作　　者：魏胜华[1,2] 钱伟[1] 孟娜[1,2] 朱必玉 周清华[1] 李琪[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000 [2]微生物发酵安徽省工程研究中心,安徽芜湖241000

出　　处：《精细化工》2016年第5期530-535,共6页Fine Chemicals

基　　金：安徽省大学生创新创业训练计划项目(201510363073)~~

摘　　要：为了提高苦杏仁β-葡萄糖苷酶的纯化度和酶活回收率,采用三相法从苦杏仁粗酶提取液中制备β-葡萄糖苷酶。分别研究了有机溶剂种类及浓度、硫酸铵饱和度、p H和温度对三相分离过程的影响。结果表明:叔丁醇为最佳有机溶剂,粗提物与叔丁醇的体积比为1.0∶1.5,硫酸铵饱和度为50%,体系p H=5.0,最佳温度为25℃。在该优化条件下,苦杏仁β-葡萄糖苷酶的纯化倍数达到5.97,酶活回收率为85.7%。In order to enhance the purification degree and activity recovery of β-glucosidase,the threephase partitioning was used to purify the enzyme from bitter almond crude extract. Different parameters,such as different organic solvents and its concentration,ammonium sulfate saturation,p H and temperature,were optimized to get highest purity fold and yield. The results showed that,t-butanol was the best organic solvent,the ratio of the volume of crude extract to t-butanol was 1. 0 ∶1. 5,ammonium sulfate saturation was 50%,the p H of system was 5. 0 and the optimum temperature was 25 ℃. Under the optimized conditions,the purification factor of β-glucosidase was 5. 97 and the activity recovery was85. 7%.

关 键 词：三相分离 苦杏仁 Β-葡萄糖苷酶 分离纯化 生物工程 

分 类 号：Q814.1[生物学—生物工程]