ε-聚赖氨酸吸附条件的优化  

OPTIMIZATION OF ADSORPTION CONDITIONS OF ε-POLYLYSINE

作　　者：朱玛骁骐 张志才[1] 刘易秋伊 夏丽莉[1] 夏建伟 郭宏明 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013 [2]江苏一鸣生物股份有限公司,泰州223001

出　　处：《离子交换与吸附》2016年第2期120-133,共14页Ion Exchange and Adsorption

基　　金：国家自然科学基金(31101269)

摘　　要：以吸附率为指标,通过单因素试验比较了5种不同的树脂类型、p H值、溶质浓度和树脂量对ε-聚赖氨酸(ε-PL)的吸附效果,进一步采用响应面设计优化了HZD-3B和D155树脂的吸附条件,分析了两种树脂吸附ε-PL的吸附等温线以及吸附动力学曲线。结果表明,试验的5种树脂中,HZD-3B和D155树脂的吸附效果较好。HZD-3B最佳静态吸附条件为:溶液起始p H 8.5、起始浓度40g/L、树脂用量150g/L、温度25℃、吸附13h,ε-PL的最大吸附率是97.69%;D155最佳静态吸附条件为:溶液起始p H 8、起始浓度40g/L、树脂用量150g/L、温度25℃、吸附14h,ε-PL的最大吸附率是96.84%;等温线数据拟合均较符合Freundlich方程,动力学数据拟合均较符合二级动力学方程。发酵液验证试验结果显示,ε-PL最大吸附量与纯品吸附量基本一致。In this study, the effect of four factors on the adsorption of e-polylysine （ε-PL） was evaluated with adsorption efficiency as an index. These factors included 5 types of cation-exchange resins, pH, concentration of adsorbate, dosage of resin and contact time. Then, the adsorption conditions of e-PL on HZD-3B or D 155 resin were optimized by response surface methods, and adsorption isotherm and dynamics equation were analyzed. Finally, the optimal conditions were verified by the fermentation broth containing ε-PL. The conclusions were obtained that （1） among the 5 types of resins tested, HZD-3B and D155 possessed higher adsorption efficiency in contrast with other 3 types of resins; （2） the optimal conditions of HZD-3B resin was as follows： pH was 8.5, the initial concentration of e-PL was 40g/L, the dosage of resins was 150g/L, and adsorption time was 13h. Under the optimal conditions, the adsorption efficiency of e-PL was 97.69%; （3） the optimal conditions of D155 resins was as follows： pH was 8.0, the initial concentration of e-PL was 40g/L, the dosage of resins was 150g/L, and adsorption time was 14h. Under the optimal conditions, the adsorption efficiency of ε-PL was 96.84%; （4） the data of adsorption experiments were fitted well by Freundlich equation and Pseudo-second order kinetics equation; （5） under the optimal conditions, the maximum adsorption capacity ofe-PL in broth was approximated to that of in pure ε-PL solution.

关 键 词：Ε-聚赖氨酸 离子交换树脂 静态吸附 响应面法 优化 吸附等温线 吸附动力学 

分 类 号：O647.316[理学—物理化学]