乙酰木聚糖酯酶协同木聚糖酶降解木聚糖的研究  

Combinative Degradation of Xylan with Acetyl Xylan Esterase and Xylanase

作　　者：朱天地 何瑶[1] 杨标[2] 刘加驰 邬敏辰[2] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学无锡医学院,江苏无锡214122

出　　处：《食品与生物技术学报》2016年第4期375-380,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31101229)

摘　　要：探讨了宇佐美曲霉(Aspergillus usamii)乙酰木聚糖酯酶和第10、11家族木聚糖酶之间的协同作用。利用作者所在实验室构建保藏的3株工程酵母Pichia pastoris GS115/Auaxe、GS115/Auxyn11A和GS115/Auxyn10A进行甲醇诱导发酵,分别获得重组乙酰木聚糖酯酶和木聚糖酶。在木聚糖酶最适p H、40℃、料液质量体积比1 g∶60 m L、水解2 h的条件下分别研究了不同加酶量作用于小麦麸皮时生成的还原糖量。结果表明,乙酰木聚糖酯酶与第11家族木聚糖酶有更好的协同作用,并在添加量比(酶活力比)为5∶1时所测协同效果最好,还原糖生成量较木聚糖酶单独作用增加了46%。因此,在乙酰木聚糖酯酶的作用下,木聚糖上乙酰基的去除,对提高木聚糖酶对木聚糖的水解效率具有重要作用。The synergetic effect of the recombinant acetylxylan esterase and xylanase from Aspergillus usamii （abbreviated as reAuAxe, reAuXyn11A, reAuXyn10A, respectively） was investigated. The fermentation of Pichia pastoris GS 115/A uaxe, GS 115/A uxyn 11A and GS115/Auxyn 10A was carried out using methanol as inducer and reAuAxe, reAuXynllA and reAuXynl0A were successfully expressed. The amounts on the release of reducing sugar were optimized with enzyme concentration when incubated for 2 h at the optiraal pH under 40 ℃ with the solid-liquid ratio of 1 g：60 mL. A better synergistic effect was found when acetylxylan esterase and reAuXynl 1A were combined. Comparing to use xylanase alone, the released reduce sugar could increase by 46% when reAuAxe and reAuXyn 11A were added with a ratio of 5：1. The removal of the O-acetyl groups from the xylose residues by acetylxylan esterase was necessary and important for the efficiency improvement ofxylan hydrolysis.

关 键 词：乙酰木聚糖酯酶 木聚糖酶 协同作用 木聚糖 

分 类 号：Q55[生物学—生物化学]