挤压超声联用提取的米糠多糖结构研究  

Research on Structure of Rice Bran Polysaccharide by Extrusion in Conjunction with Ultrasound

作　　者：王霞[1] 崔晓彤[2] 李凡姝[1] 许青[1] 杨柳[1] 肖志刚[1,3] 

机构地区：[1]沈阳师范大学粮食学院,辽宁沈阳110034 [2]沈阳师范大学化学化工学院,辽宁沈阳110034 [3]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《农产品加工（下）》2016年第4期16-19,27,共5页Farm Products Processing

基　　金：国家星火计划重点项目(2015GA650007);辽宁省"百千万人才工程"资助项目(2013921038);沈阳市科技创新专项资金项目(F14-104-3-00)

摘　　要：将米糠多糖粗液通过乙酸锌-亚铁氰化钾法脱除蛋白质得到的米糠多糖,用超滤技术分离纯化得到抗氧化活性最为显著的米糠多糖(RBS-Ⅱ)。采用高效液相凝胶渗透色谱法、光谱分析以及透射电镜研究了RBS-Ⅱ的结构。结果表明,米糠多糖的分子量分布比较均匀;红外光谱分析可知,多糖中存在α-糖苷键和β-糖苷键2种构型,含有酸性基团和蛋白质基团;通过透射电镜观察可知,多糖在水溶液中多个糖链缠绕成股,每条多糖链自身发生扭曲缠绕,进而形成不同形状的聚集体。In this paper, the crude rice bran polysaccharide as the material is studied that the removal of the rice bran polysaccharide protein in it by the method of zinc acetate-potassium ferrocyanide. And use high performance liquid gel permeation chromatography, spectroscopy and transmission electron microscopy to study the antioxidant activity of the most significant structural RBS- Ⅱ. The results show that the molecular weight of rice bran polysaccharide is more evenly distributed. Polysaccharides contain two conformations of α-glycoside bonds and β-glycoside bonds, respectively containing an acidic group and a protein group by infrared analysis. The experiment shows a plurality of sugar chains wound into strands in the aqueous solution, each polysaccharide chain wrapped and distorted, thereby formed aggregates of different shapes through a transmission electron microscope （TEM） .

关 键 词：米糠多糖 高效液相 光谱 透射电镜 超滤 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]