血红蛋白综合实验的设计与实践  

Design and practice of comprehensive experiments based on hemoglobin

作　　者：黄小花[1] 邓雅斌[1] 李东辉[1] 郑红花[1] 

机构地区：[1]厦门大学医学院基础医学实验教学中心,福建厦门361005

出　　处：《实验室科学》2016年第2期16-19,共4页Laboratory Science

基　　金：福建省科技重点项目(项目编号:2012Y0081)

摘　　要：为不断完善医学生物化学与分子生物学实验课程,实现培养创新性人才的教学目标,在本科生课程中设计以血红蛋白为实验对象,通过蛋白提取、层析纯化、SDS-PAGE电泳分离鉴定及血红蛋白与高铁血红蛋白分子吸收光谱的绘制等内容的医学生物化学综合性实验。教学实践结果表明:该实验具有可行性,学生完成情况良好;适合本科生系统完整地学习蛋白提取、纯化、鉴定及功能的实验方法,具有很强的综合性,获得良好的教学效果。To consummate the construction of medical biochemistry experimental course and cultivate of innovative talents, this study takes the hemoglobin as experimental subject to design a set of integrat- ed biochemical experiments, including extraction, gel chromatography purification, SDS-PAGE sepa- ration and acquirement of absorption spectra. The teaching practice results show that the designed ex- periments are feasible and the teaching effect is encouraging. The comprehensive experiments are suit- able for the undergraduates to study the experimental methods on protein purification and identification.

关 键 词：血红蛋白 实验教学 教学改革 

分 类 号：G482[文化科学—教育学]