双波长高效液相色谱梯度洗脱法同时测定黎峒丸中4种成分含量  

Simultaneous Determination of Four Kinds of Component Contents in Litong Pill by Double-wavelength HPLC

作　　者：孔沈燕[1] 李娴[1] 

机构地区：[1]河南中医药大学信息技术学院,郑州450046

出　　处：《医药导报》2016年第5期514-517,共4页Herald of Medicine

基　　金：国家自然科学基金青年基金资助项目(81102808)

摘　　要：目的建立双波长高效液相色谱（HPLC）梯度洗脱法同时测定黎峒丸中新藤黄酸、藤黄酸、儿茶素和表儿茶素含量。方法采用HPLC法,C18色谱柱（250 mm×4.6 mm,5μm）;流动相A为甲醇-乙腈（4∶1）,流动相B为0.2%磷酸溶液,梯度洗脱;流速1.1 m L·min^-1;检测波长360 nm（新藤黄酸和藤黄酸）,280 nm（儿茶素和表儿茶素）。结果根据回归方程,4种成分线性范围分别为新藤黄酸5.85-117.00μg·mL^-1（r=0.999 5）、藤黄酸8.95-179.00μg·mL^-1（r=0.999 8）、儿茶素6.90-138.00μg·mL^-1（r=0.999 7）、表儿茶素5.30-106.00μg·mL^-1（r=0.999 9）;平均加样回收率（n=6）分别为新藤黄酸97.82%（RSD=1.21%）、藤黄酸98.72%（RSD=1.30%）、儿茶素96.96%（RSD=0.84%）、表儿茶素99.26%（RSD=1.46%）。结论该方法操作简便、准确,重复性好,可用于黎峒丸的质量控制。Objective To develop a double-wavelength HPLC method for the simultaneous determination of neogambogic acid,gambogic acid,（ ＋）-catechin and L-epicatechin in Litong pill. Methods The quantitative analysis was carried out on C18 column（250 mm×4.6 mm,5 μm）.A linear elution of methanol-acetonitrile（4： 1）and 0.2% phosphoric acid solution was adopted at the flow rate of 1.1 mL·min^-1 ,with the detection wavelength set at 360 nm for neogambogic acid and gambogic acid,and at 280 nm for（＋）-catechin and L-epicatechin. Results The linear ranges of neogambogic acid,gambogic acid,（＋）-catechin and L-epicatechin were 5.85-117.00 μg·mL^-1（r= 0.999 5）,8.95-179.00 μg·mL^-1（r= 0.999 8）,6.90-138.00 μg·mL^-1（r= 0.999 7）,and 5.30-106.00 μg·mL^-1（r = 0.999 9）,respectively.The average recoveries were 97.82%（RSD= 1.21%）,98.72%（RSD = 1.30%）,96.96%（RSD = 0.84%）and 99.26%（ RSD = 1.46%）for neogambogic acid, gambogic acid,（＋）-catechin and L-epicatechin,respectively. Conclusion The method is simple,accurate,reproducible and may be used for the simultaneous determination of neogambogic acid,gambogic acid,（＋）-catechin and L-epicatechin in Litong pill.

关 键 词：黎峒丸 新藤黄酸 藤黄酸 儿茶素 表儿茶素 藤黄 儿茶 含量测定 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]