生物节律基因NPAS2在结直肠癌组织中的表达及意义  

Expression of circadian gene NPAS2 in colorectal cancer and its prognostic significance

作　　者：杨少芬[1] 许鸣[2] 杨海云[2] 李佩琴[1] 池秀芳[3] 

机构地区：[1]广州医科大学卫生职业技术学院病理教研室,广东广州510925 [2]广东省第二人民医院消化内科,广东广州510317 [3]广东省妇幼保健院新生儿科,广东广州510010

出　　处：《南方医科大学学报》2016年第5期714-718,共5页Journal of Southern Medical University

摘　　要：目的检测生物节律基因NPAS2在结直肠癌组织及相应癌旁组织中的表达情况;明确NPAS2的表达与患者临床病理学参数之间的相关性,分析NPAS2表达与结直肠癌患者5年生存率之间的相关性,明确其在判断结直肠癌患者预后中的作用。方法采用RT-q PCR检测NPAS2 m RNA在40例结直肠癌及癌旁新鲜组织中的表达;采用免疫组织化学SABC法检测NPAS2蛋白在120例肿瘤及癌旁石蜡标本中的表达;利用其中78例随访患者资料,K-M生存法分析NPAS2蛋白表达水平与患者生存率之间的相关性。结果 RT-q PCR结果提示,结直肠癌中NPAS2 m RNA表达显著低于相对应的癌旁组织(P<0.01);免疫组织化学结果提示,NPAS2蛋白表达在结直肠癌组织中的阳性率为19.2%,显著低于癌旁中的62.5%(P<0.05)。NPAS2的低表达与结直肠癌的肿瘤大小、淋巴结转移及TNM分期密切相关(P<0.05),而与患者年龄、性别、分化、浸润深度及远处转移无显著相关性(P>0.05)。K-M生存分析结果表明,NPAS2高表达组患者5年生存率显著高于低表达组,差异具有统计学意义(P<0.01)。结论NPAS2在结直肠癌组织中低表达,且与肿瘤部分恶性生物学行为及患者5年生存率密切相关,提示检测NPAS2的表达对预测结直肠癌患者预后具有重要意义。Objective To study the expression of NPAS2 in colorectal cancer （CRC） and analyze its relationship with the clinicopathological parameters and prognosis of the patients. Methods Real-time q-PCR was used to detect the expression of NPAS2 mRNA in 40 fresh CRC tissues and paired adjacent tissues;immunohistochemistry was used to detect the expression of NPAS2 protein in 120 paraffin-embedded tumor and adjacent tissues. The relationship between NPAS2 expression level and the 5-year survival rate of 78 CRC patients with follow-up data were analyzed with Kaplan-Meier survival analysis. Results Compared with the adjacent tissues, fresh CRC tissue expressed significantly lower NPAS2 mRNA levels （P〈0.01）. Among the paraffin-embedded CRC tissues, 19.2%were positive for NPAS2 expression, as compared to a much higher rate of 62.5%in the adjacent tissues （P〈0.05）. The expression of NPAS2 was correlated with the tumor size, lymph node metastasis and TNM stages （P〈0.05） but not with the patients＇ gender, age, distant tumor metastasis, differentiation, or invasion. Patients with high NPAS2 expression levels had a significantly higher 5-year survival rate than those with low NPAS2 expressions （P=0.0001）. Conclusion NPAS2 is down-regulated in CRC and closely correlated with the malignant biological behavior of the tumor and 5-year survival of the patients, suggesting its value in predicting the prognosis of the CRC patients.

关 键 词：结直肠癌 生物钟 生存率 NPAS2 

分 类 号：R735.34[医药卫生—肿瘤]