麻竹叶总黄酮对毛尖茶叶脂氧合酶的抑制性  

Inhibitory Effects of Flavonoids in Leaves of Dendrocalam Latiflorus on the Activity of Lipoxygenase from Maojian Tea Leaves

作　　者：吴桂玲[1] 吴艳玲[2] 杨再波[1] 刘品祯[1] 

机构地区：[1]贵州省黔南民族师范学院化学与化工系,贵州都匀558000 [2]信阳师范学院华锐学院,河南信阳464000

出　　处：《食品研究与开发》2016年第8期29-33,共5页Food Research and Development

基　　金：2013年度贵州省科学技术厅;黔南州科学技术和知识产权局;黔南民族师范学院联合基金计划项目(黔科合J字LKS[2013]17号)

摘　　要：采用正交试验法,以总黄酮含量为考察指标,对麻竹叶的总黄酮提取条件进行优选。结果表明,乙醇体积分数80%、提取时间为2 h、料液比为1∶25(g/m L),提取效率较好。最佳工艺条件下黄酮提取率为1.39%。并且研究了提取出的麻竹叶黄酮化合物对毛尖茶叶脂肪氧合酶LOX的抑制作用,并与人工合成抗氧化剂BHA、BHT和V_C的抑制作用进行比较。麻竹叶中黄酮化合物和BHA、BHT及V_C对LOX都有抑制作用,IC_(50)分别为29.478、10.938、9.049、9.04μg/m L。麻竹叶中黄酮化合物对毛尖茶叶脂氧合酶有一定的抑制作用。The optimizing extraction condition on the basis of flavonoids were determined by orthogonal design.The results showed that the optimal conditions were as follows：80 %（volume fraction）ethanol,extraction time at 2 h,1 ∶ 25（g/m L）solid-liquid ratio,the yield of total flavonoids on the optimal conditions was 1.39 %. The inhibitory effects of flavonoids from leaves of dendrocalam latiflorus on the activity of lipoxygenase from Maojian tea leaves were also studied. And compared with same concentration of BHA,BHT and V_C. Flavonoids from leaves of dendrocalam latiflorus,BHA,BHT and V_c all have inhibitory effects on the activity of lipoxygenase from Maojian tea leaves,IC_（50） were 29.478,10.938,9.049,9.04 μg/m L.The flavonoids from leaves of dendrocalam latiflorus had certain inhibition on lipoxygenase from Maojian tea leaves.

关 键 词：麻竹叶 总黄酮 正交试验 毛尖茶叶 脂肪氧合酶 抑制作用 

分 类 号：TS201.25[轻工技术与工程—食品科学]