D-101大孔吸附树脂纯化玉米须总黄酮工艺研究  

Purification process of total flavonoid from Stigma maydis by D-101 macroporous adsorption resins

作　　者：包京姗[1] 韩凤波[2] 毕博[2] 杨世海[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]吉林农业科技学院,吉林吉林132101

出　　处：《中国酿造》2016年第5期171-174,共4页China Brewing

基　　金：吉林农业科技学院中药学省级重点学科培育资助项目(吉农院合字2014第z016号)

摘　　要：通过超声波提取玉米须总黄酮,采用D-101大孔树脂进行分离纯化,得到最佳工艺参数:5 g大孔吸附树脂,玉米须总黄酮上样液质量浓度7 mg/m L,体积1 m L,以HCl调节p H=3,体积分数60%乙醇洗脱,洗脱体积5 BV,流速1.0 m L/min。在此条件下,玉米须总黄酮的纯度由41.35%提高到69.20%。Total flavonoids were extracted from Stigma maydis by ultrasonic extraction, and then they were separated and purified by D-101 macroporous adsorption resin. The optimal extraction condition was that D-101 macroporous adsorption resin 5 g, samples concentration 7.0 mg/ml, volume 1 ml, then adjust to pH 3 with HCI, elute with concentration 60% ethanol, with elute colume 5 BV and velocity 1.0 ml/min. The purification degree of total flavonoids in S. maydis increased from 41.35% to 69.20%.

关 键 词：D-101大孔吸附树脂 玉米须 总黄酮 纯化 

分 类 号：R284.2[医药卫生—中药学]