产耐高温β-葡聚糖酶菌株的筛选与初步鉴定  

Screening and Preliminary Identification of Thermostable β-glucanase Producing Strain

作　　者：孙珂[1] 王亚楠[1] 李荣贵[1] 程殿林[1] 

机构地区：[1]青岛大学生命科学学院,山东青岛266071

出　　处：《齐鲁工业大学学报》2015年第4期11-15,共5页Journal of Qilu University of Technology

基　　金：青岛市科技计划发展项目(12-1-4-2-1-jch);山东省教育厅科技项目(J07YJ19-1)

摘　　要：在啤酒酿造过程中,β-葡聚糖被内源性β-葡聚糖酶部分降解。目前,啤酒发酵工业采用添加β-葡聚糖酶的方式来降解剩余的β-葡聚糖。采用含有刚果红的分离平板筛选出一株能够在50~60℃下产β-葡聚糖酶的细菌,并对其进行了初步鉴定。结果表明,该菌为Bacillus subtilis,命名为Bacillus subtilis strain SK-1,55℃下,该菌产生的β-葡聚糖酶活力最高,可达113.41 U/mL。During the beer brewing process,β-glucan could be degradated by endogenous β-glucanases partialy. Currently,adding β-glucanase to degrade the remaining β-glucan was adopted in the beer fermentation industry. In this study,using the isolation plates contaning Congo red dye,a high-yield bacterial strain with higherβ-glucanase yield under the temperature of 50 ~60 ℃ was screened and preliminary identified. The results showed that the strain belonged to Bacillus subtilis and was named as Bacillus subtilis strain SK-1 . The enzymatic activity of β-glucanase could reach the highest under the temperature of 55 ℃ with enzyme unit of 113. 4 U/mL.

关 键 词：Β-葡聚糖酶 鉴定 筛选 枯草芽孢杆菌 

分 类 号：Q93[生物学—微生物学]