雀嘴茶中咖啡酰熊果苷提取工艺的响应面法优化  

Optimizing the extraction of 6′-O-caffeoylarbutin from Vaccinium dunalianum by response surface methodology

作　　者：李娜[1] 但汉龙[1] 刘云[1] 罗旭璐 贺鹏[1] 阚欢[1] 赵平[1] 

机构地区：[1]西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,云南昆明650224

出　　处：《西北农林科技大学学报（自然科学版）》2016年第6期173-180,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(21462040;31460076);西南林业大学科技创新基金项目(C1463)

摘　　要：【目的】确定雀嘴茶中6′-O-咖啡酰熊果苷(CA)超声辅助提取的最佳工艺。【方法】采用超声辅助法提取雀嘴茶中的CA,通过单因素试验考察甲醇体积分数(50%,60%,70%,80%,90%)、液(mL)料(g)比(8∶1,10∶1,12∶1,14∶1,16∶1)、超声时间(10,15,20,25,30min)、提取次数(1,2,3,4,5次)对CA提取率的影响,并利用响应面法优化CA的超声辅助提取工艺。【结果】超声辅助法提取CA的理论最佳工艺条件为:甲醇体积分数72.87%、液料比15.87∶1、超声时间18.96min、提取次数3.63次,CA提取率的理论预测值可达到23.12%。根据实际操作的需要,将提取工艺条件修正为甲醇体积分数73%、液料比16∶1、超声时间19min、提取次数4次,在此条件下CA实际提取率为23.03%,与理论值较为接近。【结论】采用响应面法优化雀嘴茶中CA超声辅助提取的最佳工艺完全可行。[Objective] This study aimed to determine the optimal ultrasonicassisted extraction of 6＇-O- caffeoylarbutin （CA） from Vaccinium dunalianurn buds. [Method] Ultrasonic-assisted extraction method was used to extract CA from V. dunalianum buds. The effects of methanol concentration （50%, 60%, 70%,80%,and 90%）,liquid-material ratio （8： 1,10： 1,12：1,14： 1,and 16： 1）,ultrasonic time （10,15, 20,25, and 30 rain）, and extraction times （1 , 2,3,4, and 5） on the extraction rate of CA were investigated by single factor experiments and the extraction process was optimized using response surface methodology. [Result] The theoretically optimum ultrasonic-assisted extraction conditions for CA were. methanol concentration 72.87 %, liquid-material ratio 15.87： 1, ultrasonic time 18.96 min, and extraction number 3.63 times. Under this optimal condition,the predicted extraction rate of CA was 23.12%. According to the actual experiment, the above parameters were modified to methanol concentration 73 %, liquid-material ratio16：1, ultrasonic time 19 rain, and extraction number 4 times. The actual extraction rate of CA was 23.03% ,which was close to the theoretical value. [Conclusion] It is feasible to use response surface method for optimization of ultrasonic-assisted extraction of CA from V. dunalianum buds.

关 键 词：雀嘴茶 6'-O-咖啡酰熊果苷 超声辅助提取 响应面法 

分 类 号：Q946.839[生物学—植物学] S571.109[农业科学—茶叶生产加工]