Bumetanide promotes neural precursor cell regeneration and dendritic development in the hippocampal dentate gyrus in the chronic stage of cerebral ischemia  

作　　者：Wang-shu Xu Xuan Sun Cheng-guang Song Xiao-peng Mu Wen-ping Ma Xing-hu Zhang Chuan-sheng Zhao 

机构地区：[1]Department of Neurology, First Affiliated Hospital of China Medical University [2]Neuroinfection and Neuroimmunology Center, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University [3]Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University [4]Department of Neurology, Benxi Central Hospital of China Medical University [5]Department of Medical Genetics, School of Basic Medicine, Peking University

出　　处：《Neural Regeneration Research》2016年第5期745-751,共7页中国神经再生研究（英文版）

摘　　要：Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic stage of cerebral ischemia. We established a rat model of cerebral ischemia by injecting endothelin-1 in the left cortical motor area and left corpus striatum. Seven days later, bumetanide 200 μg/kg/day was injected into the lateral ventricle for 21 consecutive days with a mini-osmotic pump. Results demonstrated that the number of neuroblasts cells and the total length of dendrites increased, escape latency reduced, and the number of platform crossings increased in the rat hippocampal dentate gyrus in the chronic stage of cerebral ischemia. These findings suggest that bumetanide promoted neural precursor cell regeneration, dendritic development and the recovery of cognitive function, and protected brain tissue in the chronic stage of ischemia.Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic stage of cerebral ischemia. We established a rat model of cerebral ischemia by injecting endothelin-1 in the left cortical motor area and left corpus striatum. Seven days later, bumetanide 200 μg/kg/day was injected into the lateral ventricle for 21 consecutive days with a mini-osmotic pump. Results demonstrated that the number of neuroblasts cells and the total length of dendrites increased, escape latency reduced, and the number of platform crossings increased in the rat hippocampal dentate gyrus in the chronic stage of cerebral ischemia. These findings suggest that bumetanide promoted neural precursor cell regeneration, dendritic development and the recovery of cognitive function, and protected brain tissue in the chronic stage of ischemia.

关 键 词：nerve regeneration cerebral ischemia BUMETANIDE Na＋-K＋-2Cl- cotransporter 1 hippocampal dentate gyrus neurogenesis neuralprecursor cells dendritic development cognitive function neural regeneration 

分 类 号：R743.3[医药卫生—神经病学与精神病学]