Smad4和Ras在外阴鳞状细胞癌中的表达及意义  

Expressions of Smad4 and Ras in Vulvar Squamous Cell Carcinoma and the Clinical Significance

作　　者：王艳实 吴琼[1] 王晨[1] 赵云霞[1] 武昕[1] 

机构地区：[1]中国医科大学附属第一医院妇科,沈阳110001

出　　处：《中国医科大学学报》2016年第6期506-509,513,共5页Journal of China Medical University

基　　金：国家自然科学基金(30973190);辽宁省科学技术计划(2014021057)

摘　　要：目的探讨Smad4和Ras在外阴鳞状细胞癌（VSCC）中的表达情况,分析两者与VSCC发生发展的关系。方法采用免疫组化方法检测Smad4和Ras在VSCC中的表达情况,并结合临床病理资料进行分析。结果 Smad4在VSCC的表达明显低于正常外阴皮肤（P〈0.05）,在VSCCⅢ期中表达明显低于Ⅰ~Ⅱ期（P〈0.05）,中低分化的表达明显低于高分化（P〈0.05）,有淋巴结转移的表达明显低于无淋巴结转移（P〈0.05）。Ras蛋白在VSCC的表达明显高于正常外阴皮肤（P〈0.05）,在VSCCⅢ期中表达明显高于Ⅰ~Ⅱ期（P〈0.05）,有淋巴结转移的表达明显高于无淋巴结转移（P〈0.05）,VSCC低、中分化以及高分化之间无统计学差异（P〉0.05）。Spearman相关性分析结果表明：在VSCC中Smad4和Ras的表达呈负相关。结论 Smad4的低表达及Ras的高表达可能与VSCC的发生、发展有关。Objective To investigate the expression of Smad4 and Ras in vulvar squamous cell carcinoma（VSCC）,and explore their association with development of disease. Methods Immunohistochemical staining was conducted to detect the expression of Smad4 and Ras in VSCC. Then the relationship between the expression of Smad4,Ras protein and clinical and pathological data of patients with VSCC were analyzed. Results The expression of Smad4 in VSCC was significantly lower than normal vulva skin tissue（P〈0.05）. Smad4 expression in VSCC tissue of stage Ⅲ was significantly lower than that of stage Ⅰ-Ⅱ（P〈0.05）. The expression of Smad4 in the moderately and poorly differentiated VSCC was lower than those well differentiated VSCC（P〈0.05）. The expression of Smad4 in cases with lymph node metastasis was lower than those without lymph node metastasis（P〈0.05）. The expression of Ras in VSCC was significantly higher than normal vulva skin tissue and the difference was statistically significant（P〈0.05）. The expression of Ras was higher in Ⅲ stage than in Ⅰ-Ⅱ stage（P〈0.05）. The expression of Ras in cases with lymph node metastasis was higher than those without lymph node metastasis（P〈0.05）. Ras expression had no statistically significant difference between the poorly and moderately differentiated VSCC and the highly differentiated ones（P〉0.05）. The Spearman correlation analysis showed that Smad4 and Ras expression was negatively correlated in vulva squamous cell carcinoma. Conclusion The expression of Smad4 and Ras was associated with occurrence and development of the VSCC.

关 键 词：外阴鳞状细胞癌 SMAD4 RAS 免疫组织化学 

分 类 号：R737.35[医药卫生—肿瘤]