食管癌细胞线粒体DNA编码区基因突变研究  

Encoding gene mutation of mitochondrial DNA in esophageal cancer cells

作　　者：楚阿兰 刘宗文[1] 田薇薇[2] 梁冰[2] 宋锐[1] 张钰浩[3] 张艳[3] 

机构地区：[1]郑州大学第二附属医院肿瘤放疗科,450014 [2]郑州大学第二附属医院肿瘤科,450014 [3]郑州大学基础医学院药理教研室,450001

出　　处：《中华实验外科杂志》2016年第5期1260-1263,共4页Chinese Journal of Experimental Surgery

基　　金：2014年度河南省医学科技攻关计划项目（201401009）;郑州市2013年第一批科技计划项目（131PCXTD628）

摘　　要：目的 探讨食管癌细胞线粒体DNA （mtDNA）编码区基因突变与食管癌发生、发展的关系.方法 分别培养3种食管癌细胞EC9706细胞、TE-1细胞、Eca109细胞以及正常食管黏膜细胞Het-1A细胞,分别提取其mtDNA,利用聚合酶链反应（PCR）扩增、纯化及直接测序,检测4种细胞中mtDNA全编码区基因突变.结果 3种食管癌细胞的mtDNA编码区中共发现39个突变位点,Het-1A细胞中共检测到11个碱基改变位点;4种细胞mtDNA编码区碱基置换大部分是A-G、C-T之间的碱基改变.3种食管癌细胞中发生在蛋白编码区的错义突变有11个,除A8860G、A15326G这两个位点同时出现在正常食管黏膜细胞,剩余9个仅在癌细胞中出现,分别为G6366A、A8701G、A10086G、A10398G、A12358G、A13105G、C14766T、A15311G、A15824G,分别位于细胞色素C氧化酶亚基1（COX1）、三磷酸腺苷合成酶6（ATP6）、烟酰胺腺嘌呤二核苷酸脱氢酶3（ND3）、ND3、烟酰胺腺嘌呤二核苷酸脱氢酶5（ND5）、ND5、细胞色素B（CytB）、CytB、CytB.结论 食管癌细胞mtDNA编码区基因突变与食管癌发生、发展有关.Objective To investigate the relationship of mitochondrial DNA （mtDNA） encoding gene mutation with the occurrence and development of esophageal cancer.Methods MtDNA was extracted in three kinds of human esophageal cancer cell lines EC9706,TE-1 and Eca109,and normal esophagus cell line,Het-1A.All of the encoding genes were sequenced.Results There were 39 mutations in three kinds of esophageal cancer cells,and 11 mutations in Het-1A cells.Most of the mutations were A-G or C-T.A total of 11 protein coding mutations have been identified.Except A8860G and A15326G,the two mutations appeared in normal esophageal cells at the same time,and the remaining 9 mutations only appeared in cancer cells （G6366A,A8701G,A10086G,A10398G,A12358G,A13105G,C14766T,A15311G,and A15824G）.The corresponding mutation genes were cytochrome c oxidase subunit 1 （COX1）,ATP synthase 6 （ATP6）,nicotinamide adenine dinucleotide dehydrogenase 3 （ND3）,ND3,nicotinamide adenine dinucleotide dehydrogenase 5 （ND5）,ND5,cytochromes B （CytB）,CytB,CytB.Conclusion The mutations of the mtDNA encoding gene of esophageal cancer cells were related to the occurrence and development of esophageal cancer.

关 键 词：食管癌 线粒体DNA 编码区基因 突变 

分 类 号：R735.1[医药卫生—肿瘤]