p53途径的双荧光素酶报告系统在食品致癌物筛选中的应用研究  

Carcinogen screening in food with dual luciferase assay system based on p53 pathway

作　　者：林春芳[1] 李艳[2] 郑奎城[1,2] 谢剑锋[1] 傅武胜[1] 赵康涛[1] 

机构地区：[1]福建省疾病预防控制中心(福建省人兽共患病研究重点实验室),福州350001 [2]福建医科大学公共卫生学院,福州350100

出　　处：《海峡预防医学杂志》2016年第2期4-6,9,共4页Strait Journal of Preventive Medicine

基　　金：福建省自然科学基金计划项目(No.2012J01314)

摘　　要：目的应用p53途径的双荧光素酶报告系统筛选食品中的致癌物。方法双荧光素酶试验中,采用萤火虫荧光素酶检测食品中常见的致突变性/致癌性物质的p53基因表达水平,同时以海肾荧光素酶作为内对照。结果文献报道有致突变性/致癌性的8个样品中有7个双荧光素酶试验结果为阳性,而且阳性剂量低于大多数传统毒理试验,阳性样品的荧光素酶活性有随着处理剂量的增加而增高的趋势;缺乏致突变性/致癌性文献的隐色结晶紫的荧光素酶活性仅略有增加。结论 p53途径的双荧光素酶报告系统可能是一种比较理想的高通量食品致癌物筛选系统。Objective To screen carcinogens in food with dual luciferase assay system based on p53 pathway.Methods In dual-glo luciferase assay system,firefly luminescence was carried out to detect expression levels of p53 caused by common mutagens/carcinogens in food,while Renilla luminescence was used as an internal control.Results The results of dual-glo luciferase assay system were positive for 7 of 8 reported mutagens/carcinogens.Dual luciferase assay was more sensitive than most traditional toxicological assays,and higher treatment doses resulted in more active luciferases.The luciferase activity caused by leucocrystal violet,a chemical without mutagenic/carcinogenic reports,only slightly increased.Conclusion Dual luciferase assay system based on p53 pathway may be suitable for screening carcinogens in food.

关 键 词：食品检测 遗传毒性 高通量筛选 双荧光素酶试验 P53基因 致癌物 

分 类 号：R155.5[医药卫生—营养与食品卫生学]