脱落酸对采后番茄果实损伤愈合的作用  

Effect of abscisic acid on wound-healing process in postharvest tomato fruit

作　　者：陶晓亚[1] 李家寅[1] 茅林春[1] 

机构地区：[1]浙江大学食品科学与营养系浙江省农产品加工重点实验室,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2016年第3期321-326,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(31372113)

摘　　要：采用脱落酸(abscisic acid,ABA)及其合成抑制剂氟啶酮(fluridone,FLD)进行处理,研究ABA对采后番茄果实的愈伤能力及酶活性的影响。绿熟期樱桃番茄表皮切伤处理后,分别用ABA和FLD真空渗透处理(0.07MPa,5min),于黑暗条件下(温度20℃,相对湿度90%)贮存愈伤,每天取样测定与损伤愈合相关的果实失重率、自发荧光强度、苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)和过氧化物酶(peroxidase,POD)活性。结果表明:在愈伤过程中,对照组番茄果实ABA含量先降低后升高,而ABA组始终保持较高水平,FLD组则始终维持较低水平;番茄果实失重率均逐渐升高,ABA组始终低于对照组,FLD组则始终高于对照组;损伤部位细胞自发荧光强度逐渐增强,ABA组始终强于对照组(尤其在第3天以后更为显著),FLD组则始终弱于对照组;PAL活性先升高后降低,在第3天达到峰值,而POD活性则先降低后升高,2种酶活性均表现为ABA组高于对照组,FLD组低于对照组。以上结果说明,ABA具有促进采后番茄果实损伤愈合的作用。该研究将为采后果蔬损伤愈合机制的研究提供理论基础。The effect of abscisic acid（ABA）on wound-healing capability and related enzyme activities were investigated in postharvest cherry tomato fruits treated by ABA and fluridone（FLD,an inhibitor of ABA）.After wounding at equator with a sterilized scalpel,fruits were immersed in 1.0mmol/L ABA,0.1mmol/L FLD and deionized water（control,CK）under vacuum（0.07 MPa,5 min）,respectively.Treated fruits were allowed to wound-heal at 20 ℃ and 90% relative humidity in dark.Fruit weight loss,autofluorescence intensity,phenylalanine ammonia-lyase（PAL）and peroxidase（POD）activities were determined in wound tissues every day.The results indicated that ABA content decreased within the first two days followed by a slight increase in the control,but maintained at high levels in ABA group and low levels in FLD group.Fruit weight loss increased gradually in all groups during storage.However,the weight loss was more and faster in FLD group,but less andslower in ABA group in contrast with the control.The intensity of autofluorescence increased during woundhealing.Compared with the control,ABA group exhibited an increase in the intensity of autofluorescence especially after 3dof storage,while FLD group depicted a reduced intensity.PAL activity increased after wounding,and reached the maximum at the third day and then decreased,while POD activity decreased after wounding and then increased in all groups during wound-healing.PAL and POD activities were enhanced by ABA application while inhibited by FLD treatment compared with the control.In conclusion,ABA promotes wound-healing process in postharvest tomato fruits.This study provides theoretical basis for the research of wound-healing mechanism in postharvest fruit and vegetable.

关 键 词：脱落酸 采后 番茄 损伤愈合 苯丙氨酸解氨酶 过氧化物酶 

分 类 号：S641.2[农业科学—蔬菜学]