三孢布拉霉高产β-胡萝卜素菌株选育  

Breeding of the Blakeslea trispora strain with high β-carotene production

作　　者：刘星[1] 刘姗姗[1] 殷海松 乔长晟[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津市现代职业技术学院,天津300350

出　　处：《饲料工业》2016年第10期47-50,共4页Feed Industry

基　　金：天津市科技计划项目[14ZCZDTG00025]

摘　　要：试验旨在研究利用三孢酸筛选高产β-胡萝卜素菌株。通过对负菌进行紫外诱变处理,利用三孢酸进行平板初筛,摇瓶复筛,遗传稳定性分析后,最终从多株目的菌株中选育出一株产β-胡萝卜素量较为稳定,产量达到694.26 mg/l的菌株,较出发菌株产量提高52.01%。利用此育种方法筛选高产β-胡萝卜素菌株具有较高的可行性。In this paper, a new mutation breeding method using trisporic acid to obtain the mutant withhigh production of β-carotene was studied. The original（-） strain was treated with UV（ultraviolet radi-ation）and then mutants with higher production of β- carotene was selected from selection mediumplates and fermentation of β-carotene. After being selected from selection medium plates, fermentationof β-carotene test and genetic stability test, the mutant with the highest production of β-carotene wasobtained from the target mutants with the capacity of 694.26 mg/l, 52.01% up than that of the originalstrain. Using this method to obtain the strain with high production of β-carotene is working.

关 键 词：三孢布拉霉 诱变选育 三孢酸 Β-胡萝卜素 

分 类 号：TQ92[轻工技术与工程—发酵工程]