核黄素对厚皮甜瓜果实粉霉病和黑斑病的影响  

Effect of Riboflavin on Pink Mold and Black Spot of Muskmelon Fruits

作　　者：李灿婴[1] 葛永红[1] 吕静祎 成白苗 吴永升[2] 

机构地区：[1]渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《食品科学》2016年第12期243-247,共5页Food Science

基　　金：国家自然科学基金青年科学基金项目(31401554);渤海大学博士启动基金项目(bsqd201405)

摘　　要：研究不同浓度核黄素处理时厚皮甜瓜果实损伤接种粉红单端孢(Trichothecium roseum)和互隔交链孢(Alternaria alternata)的病斑直径,以及离体培养条件下核黄素处理对两种病原物孢子萌发和菌丝生长的影响。同时研究核黄素处理对厚皮甜瓜果实苯丙烷代谢的影响。结果表明:核黄素浓度为1.0 mmol/L时对甜瓜粉霉病和黑斑病病斑直径的抑制效果最佳。离体培养情况下核黄素处理对T.roseum和A.alternata的孢子萌发率和菌丝生长有抑制效果,其中以浓度为1.0 mmol/L效果最佳。核黄素处理显著提高了厚皮甜瓜果实过氧化物酶和苯丙氨酸解氨酶的活性,还显著提高了总酚、类黄酮、木质素的含量。由此表明,采后核黄素处理可活化苯丙烷代谢,增加相关酶活性及抗性物质的积累,从而强化果实细胞壁结构,抑制果实采后病害的发生。The effects of postharvest riboflavin dipping at different concentrations on lesion development of muskmelon fruits artificially wounded and inoculated with Trichothecium roseum and Alternaria alternata were examined. The metabolisms of phenylpropanoid pathway and the effects of riboflavin at different concentrations on mycelia growth and spore germination of T. roseum and A. alternata were also studied. The results indicated that riboflavin at 1.0 mmol/L effectively decreased the lesion diameter of muskmelon fruits inoculated with T. roseum and A. alternata. In vitro test also indicated that riboflavin at 1.0 mmol/L effectively inhibited mycelia growth and spore germination of both pathogens. It was also shown that postharvest riboflavin treatment significantly enhanced the activities of peroxidase（POD） and phenylalanine ammonia lyase（PAL）, and increased the contents of total phenolic compounds, flavonoids and lignin in muskmelon fruits. These results suggest that postharvest riboflavin treatment strengthens the cell wall through activating the phenylpropanoid pathway and enhancing the activities of defense-related enzymes and the accumulation of antifungal compounds to control postharvest diseases in fruits.

关 键 词：厚皮甜瓜 核黄素 苯丙烷代谢 采后病害 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]