接种量对螺旋霉素发酵生产的影响  

The effects of inoculum volume on spiramycin fermentation

作　　者：张义馨 张杨平 周锦敏 邓锋 

机构地区：[1]福建和泉生物科技有限公司,福建南平354200

出　　处：《发酵科技通讯》2016年第2期110-113,共4页Bulletin of Fermentation Science and Technology

摘　　要：在2 m^3中试发酵罐上探讨不同接种量对生二素链霉菌螺旋霉素发酵单位和发酵周期的影响,以最优的接种量在60 m^3发酵罐中进行产业化验证。结果表明:在2 m^3发酵罐试验中接种量为16%(体积比)且发酵周期120 h时,发酵单位达到最高即4.72 g/L。在60 m^3发酵罐上接种16%的二级种子液进行20批放大验证,平均发酵单位达4.85 g/L。In this study, the effects of inoculum volume on spiramycin titer and fermentation period of Streptomyces ambofaciens were investigated in 2 m^3 pilot fermenters, and then industrial verification was carried out in 60 m^3 fermenters with optimized inoculum volume. The results showed that spiramycin titer reached 4.72 g/L with inoculum volume of 16%（V/V） after 120 h fermentation in 2 m^3 fermenters. Twenty batches of scale-up verifications were performed in 60 m^3 fermenters by inoculating 16% of seed broth and the average titer reached up to 4.85 g/L.

关 键 词：螺旋霉素 接种量 发酵单位 验证 

分 类 号：TQ92[轻工技术与工程—发酵工程]