蛋白质组学技术及其在工业微生物研究中的应用  

Proteomic technologies and their applications in the research of industrial microbiology

作　　者：王亚军[1,2] 王超儿[1,2] 朱津[1,2] 罗希[1,2] 

机构地区：[1]浙江省生物有机合成技术研究重点实验室,浙江杭州310014 [2]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《发酵科技通讯》2016年第2期123-128,共6页Bulletin of Fermentation Science and Technology

基　　金：国家自然科学基金(21476209)

摘　　要：随着后基因组时代的到来,蛋白质组学的研究受到越来越多的关注,双向凝胶电泳和生物质谱技术是蛋白质组学研究的两大工具。微生物被广泛应用于食品、化工、医药等领域,随着这些领域的快速发展,利用蛋白质组学技术解析微生物代谢途径及调控机制越来越受到重视,从而为进一步的菌种改良提供理论依据。主要综述了蛋白质组学相关技术及其在工业微生物代谢途径与调控机制研究中的应用进展。Proteomics, based on two-dimensional electrophoresis and mass spectrum, is becoming the spotlight of life science in the post-genome era. With the fast development of industrial microbiology in food, pharmaceutical and chemical industry, proteomics technologies began to play a vital role in the research of metabolic pathways and its regulation, which provided theoretical foundation for microbial breeding. The principle, advantages and disadvantages of the widely used technologies in proteome research were reviewed, along with their applications in the metabolic engineering research of industrial microorganisms.

关 键 词：蛋白质组学 双向电泳 质谱 微生物育种 

分 类 号：Q93[生物学—微生物学]