早期子宫颈鳞状细胞癌中Livin、Apaf-1和ARID1A的表达及临床意义  

The expression of Livin, Apaf-1 and ARID1A in early stage squamous cell carcinoma of cervix and their clinical significances

作　　者：徐义荣[1] 陈振文[1] 郗彦凤[2] 张广衡[1] 张朝霞[1] 柴菲[1] 董婧妍 蔡文慧[1] 田林 

机构地区：[1]山西医科大学汾阳学院病理教研室,032200 [2]山西省肿瘤医院病理科,太原030013 [3]山西省汾阳医院病理科,032200

出　　处：《肿瘤研究与临床》2016年第5期294-299,共6页Cancer Research and Clinic

基　　金：山西省自然科学基金（2012011038-4）;山西医科大学汾阳学院院内科研项目

摘　　要：目的 研究早期子宫颈鳞状细胞癌中Livin、Apaf-1和ARID1A蛋白的表达情况,探讨其与患者临床病理特征及预后的关系.方法 应用免疫组织化学SP法检测Livin、Apaf-1和ARID1A蛋白在166例早期子宫颈鳞状细胞癌组织和50例正常子宫颈组织中的表达情况.结果 Livin和ARID1A在早期子宫颈鳞状细胞癌组织中的阳性率分别为46.9％（78/166）、64.6％（107/166）,明显高于正常子宫颈组织的12.0％（6/50）、30.0％（15/50）（P＜0.05）;而Apaf-1在子宫颈鳞状细胞癌组织中的阳性率为33.7％（56/166）,明显低于正常子宫颈组织的52.0％（26/50）（P＜ 0.05）.Livin蛋白表达与临床国际妇产科协会（FIGO）分期、局部淋巴结转移和脉管浸润呈正相关（P＜0.05）,Apaf-1与Livin表达和局部淋巴结转移呈负相关（P＜0.05）.Livin阴性组患者总生存（OS）时间长于Livin阳性组,Apaf-1阳性组患者OS时间长于Apaf-1阴性组（P＜0.05）.Livin蛋白表达和淋巴结转移是早期子宫颈鳞状细胞癌的独立预后因素（P＜0.05）.结论 Livin、Apaf-1和ARID1A蛋白在子宫颈鳞状细胞癌和正常子宫颈组织的表达存在差异;Livin阳性提示预后差,其可以作为早期子宫颈鳞状细胞癌独立预后判断指标.Objective To explore the expression of Livin,Apaf-1 and ARID1A protein in early stage squamous cell carcinoma （SCC） of cervix,and to analyze their relationships with clinicopathological characteristics and patients＆#39; prognosis.Methods SP immunohistochemistry was used to detect the expression of Livin,Apaf-1 and ARID1A protein in 166 early stage SCC tissues and 50 normal cervical tissues.Results The positive rates of Livin and ARID1A in SCC tissues were respectively 46.9 % （78/166） and 64.6 % （107/166）,which were obviously higher than those in normal cervical tissues [12 % （6/50） and 30.0 % （15/50）,P 〈 0.05].The positive rate of Apaf-1 in SCC tissues was 33.7 % （56/166）,which was lower than that in normal cervical tissues [52.0 % （26/50）,P 〈 0.05].There were positive correlation between the expression of Livin and clinical FIGO stage,local lymph node metastasis and vascular infiltration （P 〈 0.05）.The expression of Apaf-1 was negatively correlated with the expression of Livin and local lymph node metastasis （P 〈 0.05）.The overall survival （OS） time of Livin negative cases was longer than that of Livin positive cases,and the OS of Apaf-l positive cases was longer than the Apaf-1 negative cases,with significantly statistical difference （P 〈 0.05）.Conclusions The expression levels of Livin,Apaf-1 and ARID1A protein have significant differences between the SCC tissues and normal cervical tissues.The positive expression of Livin shows poor prognosis,and Livin can be served as the independent prognostic indicator of early stage SCC.

关 键 词：子宫颈肿瘤 癌 鳞状细胞 LIVIN APAF-1 ARID1A 预后 

分 类 号：R737.33[医药卫生—肿瘤]