高产纤维素酶木霉菌株的筛选  

Screening of High-yield Cellulase Trichoderma

作　　者：冯小飞[1] 赵宁[1] 陈玉惠[1] 

机构地区：[1]西南林业大学生命科学学院,昆明650224

出　　处：《湖北农业科学》2016年第6期1429-1432,共4页Hubei Agricultural Sciences

基　　金：云南省优势特色重点学科生物学一级学科建设项目(50097505)

摘　　要：以前期筛选获得的20株木霉菌株为试验对象,通过刚果红羧甲基纤维素钠（CMC-Na）培养基筛选出11株产纤维素酶能力较好的菌株,对其进行酶活性检测,发现菌株SS003产生的纤维素酶活性最高,并研究了温度和pH对该菌株所产的纤维素粗酶活性的影响。结果表明,SS003产生的纤维素酶最适反应温度为55～60℃,最适pH为3.0。The research was based on the 20 strains of Trichoderma which was pre-screened. 11 strains of Trichoderma with high-yield cellulose were screened out from the CMC-NaCongo red medium. The enzymatic activity was detected and the in- fluence of temperature and pH on the coarse cellulose enzyme activity was studied. The results showed that the cellulose en- zyme activity produced by strain SS003 was the highest. The optimal temperature of cellulase for the strain SS003 was 55-60 ℃ and the optimal pH was 3.0.

关 键 词：木霉 纤维素酶 酶活力 

分 类 号：Q55[生物学—生物化学]