烟碱对东方伊萨酵母HN-1乙醇发酵的影响  

Effect of nicotine on ethanol fermentation of Issatchenkia orientalis HN-1

作　　者：王风芹[1] 付晨青 谢瑶嬛 谢慧[1] 任天宝[2] 宋安东[1] 

机构地区：[1]河南农业大学生命科学学院农业部农业微生物酶工程重点实验室,河南郑州450002 [2]河南农业大学烟草学院,河南郑州450002

出　　处：《食品与发酵工业》2016年第5期56-61,共6页Food and Fermentation Industries

基　　金：河南省高校科技创新团队支持计划(15IRTSTHN014)

摘　　要：以乙醇发酵工业菌株酒精酵母1308为对照,研究了烟碱对东方伊萨酵母HN-1乙醇发酵的影响,以期为烟秆等烟草废弃物的资源化利用提供宝贵的菌种资源。结果表明,烟碱对两菌种的菌体生长具有显著的抑制作用;当烟碱添加量为0.1%~0.5%时,东方伊萨酵母HN-1和酒精酵母1308的乙醇产量分别为18.17~9.51g/L和11.95~5.42 g/L,较对照分别下降了20.81%~58.55%和40.42%~72.94%,同时1308菌株的发酵周期较对照推迟12 h;烟碱对1308菌株葡萄糖利用的抑制作用显著强于对HN-1的抑制作用。副产物结果分析表明,HN-1菌株乙酸产量为0.11~0.43 g/L,低于1308菌株的0.20~0.59 g/L;甘油产量为2.20~2.71 g/L,显著高于1308菌株的1.48~2.33 g/L。东方伊萨酵母HN-1较酒精酵母1308更适合用于烟秆等烟草废弃物生物转化乙醇的研究与生产。The effects of nicotine on the ethanol fermentation by Issatchenkia orientalis HN-1 were studied using industrial strain Saccharomyces cerevisiae 1308 as control to provide valuable strain resources for the comprehensive utilization of tobacco stalk and other tobacco waste. The results indicated that nicotine could inhibit cell growth of these two strains significantly. After addition of nicotine from 0. 1% to 0. 5%,ethanol production of Issatchenkia orientalis HN-1 and Saccharomyces cerevisiae 1308 was 18. 17 ~ 9. 51 g / L and 11. 95 ~ 5. 42 g / L,respectively,which was decreased by 20. 81% ~ 58. 55% and 40. 42% ~ 72. 94% compared with the blank without addition of nicotine. Meanwhile,the fermentation period of strain 1308 postponed 12 h compared with the blank control,and the inhibition of nicotine on glucose consumption in this group was also stronger than that treated with strain HN-1. Analysis of byproducts showed that the yield of acetic acid of strain HN-1 was 0. 11 ~ 0. 43 g / L,which was lower than that of strain1308 with the yield of 0. 20 ~ 0. 59 g / L. However,glycerol production of strain HN-1 was 2. 20 ~ 2. 71 g / L,with was significantly higher than that of strain 1308 with the production of 1. 48 ~ 2. 33 g / L. Issatchenkia orientalis HN-1 was more suitable for bioconversion of tobacco stalk and other tobacco waste into ethanol compared with Saccharomyces cerevisiae 1308.

关 键 词：酵母菌株 乙醇发酵 烟碱胁迫 耐受性 

分 类 号：TQ223.122[化学工程—有机化工] TQ920.6[轻工技术与工程—发酵工程]