甜茶素和甜茶总多酚的制备  

Preparation technology of rubusoside and total polyphenols from sweet tea

作　　者：鲍晨阳 韦万丽[1] 陈显刚 廖莉玲[1] 

机构地区：[1]贵州师范大学化学与材料科学学院,贵州贵阳550001 [2]贵州省健康茶叶科技有限公司,贵州黔南558100

出　　处：《食品与发酵工业》2016年第5期180-183,共4页Food and Fermentation Industries

基　　金：贵州省科学技术厅中药现代化攻关项目(黔科合ZY字【2012】3012号);贵阳市科技局现代药业计划项目(筑科合同[2012204]号)

摘　　要：设计了从甜茶叶同时制备甜茶素和甜茶总多酚的工艺,简化了甜茶素生产流程,提高了产率。首先将甜茶提取液用大孔树脂富集,收集柱流液,然后通过低浓度的乙醇溶液对饱和的甜茶素富集柱进行洗脱,合并洗脱液和柱流液,用大孔树脂对合并液进行纯化,得到的总多酚产品纯度可以达到约60%,产率约为10%,再用高浓度的乙醇溶液对甜茶素富集柱进行洗脱,旋干,得到甜茶素粗品,纯度约为45%,产率为7.1%。若再用聚酰胺树脂对甜茶素粗品进行精制,则纯度可达到约71%,产率为3.4%。Technology of preparing rubusoside and total polyphenols from sweet tea in the same time was designed. The technology simplified the process of preparing rubusoside and improved the yield. First of all sweet tea extract was enriched by the macroporous resin and the column flow fluid was collected. Then the saturated rubusoside enrichment column was eluted by low concentration of ethanol solution,the eluent and column flow fluid were combined and purified by macroporous resin HPD826. Column conduction was flow rate of 2 BV / h,sample volune of 2BV and 40% ethanol solution of 3 BV as eluent. Eluent was collected and concentrated by rotary evaporator and freeze-dried. The purity and yield of the total polyphenols production were 60% and 10%,respectively. Then the enrichment column was eluted again by a high concentration of ethanol solution,eluent was dried by the rotary evaporator,and crude product of rubusoside was obtained. The purity and yield of production were 45% and 7. 1%,respectively. Polyamide resin was used for refining crude product; column condition was same as HPD826. Column flow was collected and concentrated by rotary evaporator and freeze-dried. The purity of production could reach about 71%,the yield was about 3. 4%. The solvent and macroporous resin could be recycled in the whole new process flow; the technological process was simple,green,and suitable for industrial production.

关 键 词：甜茶素 甜茶多酚 制备工艺 

分 类 号：TS202.3[轻工技术与工程—食品科学]