拉曼光谱法快速检测硬糖中的诱惑红  

Rapid detection of allura red in hard candy using raman scatter spectroscopy

作　　者：陈思[1] 郭平 骆鹏杰 吴瑞梅[4] 王文君[1] 方畑 

机构地区：[1]江西农业大学食品科学与工程学院,江西南昌330045 [2]江西省出入境检验检疫局技术中心,江西南昌330002 [3]国家食品安全风险评估中心,北京100022 [4]江西农业大学生物光电及应用重点实验室,江西南昌330045

出　　处：《食品与机械》2016年第4期76-79,142,共5页Food and Machinery

基　　金：十二五国家科技支撑计划项目(编号:2012BAK17B02);江西省科技计划项目(编号:20151BBG70066)

摘　　要：采用便携式拉曼光谱仪快速检测硬糖中的诱惑红色素。分析诱惑红分子的普通拉曼谱峰与密度泛函计算理论拉曼峰,对诱惑红分子进行谱峰归属,确定诱惑红分子的6个拉曼特征谱峰:1 222,1 272,1 330,1 408,1 500和1 580cm^(-1)。由峰强较高的特征峰1 500cm^(-1)的强度,建立定量分析硬糖中诱惑红色素的标准曲线。该曲线在0.1~1.0g/kg范围内呈现良好的线性关系,其决定系数为0.995,回收率为95.99%~102.92%,相对标准偏差为3.19%~5.59%。该方法检测硬糖诱惑红色素的最低检测限低于0.1g/kg,能达到半定量检测的目的,可为拉曼光谱检测硬糖中诱惑红分子的快速检测提供参考依据。Raman spectroscopy was employed to detect the allura red in hard candy using portable raman spectrometer.After attributed and analyzed the raman peaks of allura red by density functional theory method and normal raman spectroscopy,six characteristic raman peaks were confirmed which were 1 222,1 272,1 330,1 408,1 500 and 1 580cm^（-1）,respectively.Then the standard curve was established under the characteristic raman peaks with 1 500cm^（-1）.The results indicated that the curve possessed a good linear relationship between 0.1~1.0g/kg,the determination coefficient was 0.995,average recovery of allura red ranged from 95.99% to 102.92%,and the relative standard deviation（RSD）was between 3.19%~5.59%.The limits of detection（LOD）of allura red reached to 0.1g/kg,which conformed to the national standard.These results would provide scientific reference for raman spectroscopy rapid detection,especially detected the allura red in hard candy.

关 键 词：拉曼光谱 诱惑红 硬糖 快速检测 

分 类 号：O657.37[理学—分析化学] TS247[理学—化学]