侧孢短芽孢杆菌S62-9的生物学特性研究  

A Study on the Biological Characteristics of Brevibacillus laterosporus S62-9

作　　者：王志新[1] 张丹[2] 李兴峰[1] 宁亚维[1] 张凤娇[1] 贾英民[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018 [2]河北农业大学食品科技学院,河北保定071001

出　　处：《现代食品科技》2016年第5期52-58,共7页Modern Food Science and Technology

基　　金：国家科技支撑计划(2013BAD10B03);国家农业科技成果转化项目(2013GB2A200032);河北省自然科学基金(C2014208137)

摘　　要：以实验室选育的一株侧孢短芽孢杆菌Brevibacillus laterosporus S62-9为对象,体外研究其耐热性、耐酸性、耐胆盐性、黏附性以及药敏性等生物学特性。结果表明,B.laterosporus S62-9耐热性较好,85℃水浴5 min,存活率达86.20%;人工胃液中作用4 h,p H 2.0下的存活率最低,为85.70%,p H 3.0和p H 4.0下的存活率均高于95.00%;人工肠液中作用4 h,胆盐浓度为0.1%、0.2%和0.3%时的存活率分别为93.30%,91.70%和88.30%;对模拟胃肠道消化过程的耐受性高,p H 2.0胃液处理2 h后又经0.3%胆盐处理3 h,存活率达96.00%;营养体状态的B.laterosporus S62-9对Caco-2细胞体外的粘附率为20.70 CFU/cells,黏附能力高于对照组枯草芽孢杆菌K1;对养殖中常用的10种抗生素均未产生耐药性,是安全菌株。B.laterosporus S62-9优良的体外益生菌特性符合微生态制剂菌株的选育标准和要求,为其微生态制剂的开发与应用奠定了理论基础。The thermal resistance, acid resistance, bile salt resistance, adhesion ability, antibiotic susceptibility, and other biological characteristics of Brevibacillus laterosporus strain S62-9 were studied in vitro. Strain S62-9 exhibited good thermal tolerance, with a survival rate of 86.20% after incubation in an 85 ℃ water bath for 5 min. It had the lowest survival rate（85.70%） after exposure to simulated gastric juices at p H 2.0 for 4 h, whereas the survival rates at p H 3.0 and 4.0 were both higher than 95.00%. The survival rates after 4-h treatments in 0.1%, 0.2%, and 0.3% bile-salt-stimulated intestinal fluids were 93.30%, 91.70%, and 88.30%, respectively. Strain S62-9 showed high tolerance to simulated gastrointestinal transit, where its survival rate was 96.00% after being treated with gastric juice at p H 2.0 for two hours followed by a three-hour treatment in intestinal fluid containing 0.3% bile salt. The bacterium in the trophozoite stage showed an adhesion ability of 20.70 CFU per Caco-2 cell, which was higher than that of the control strain（Bacillus subtilis K1）. Strain S62-9 had no observable resistance to 10 types of antibiotics tested and was thus considered to be a biosafe strain. These excellent probiotic characteristics of B. laterosporus S62-9 meet the standards and requirements of a good probiotic, laying a theoretical basis for its development and application as a probiotic strain.

关 键 词：侧孢短芽孢杆菌 耐受性 黏附性 药敏性 微生态制剂 

分 类 号：TS201.3[轻工技术与工程—食品科学]