响应面优化菊芋菊糖的提取工艺研究  

Extraction Process Optimization of Inulin from Helianthus tuberosus L.by Response Surface Methodology

作　　者：刘燕[1,2] 陈小银[1] 杨丽丽[1] 谢瑞[1] 何楠[1] 张继[1,2] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃特色植物有效成分制品工程技术研究中心,兰州730070

出　　处：《植物研究》2016年第4期627-633,共7页Bulletin of Botanical Research

基　　金：国家科技支撑计划(2012BAD20B06);国家自然基金(31200255)~~

摘　　要：利用单因素实验及响应面法优化确定菊芋块茎中菊糖的提取工艺。通过单因素实验筛选出液固比、提取时间、提取温度3个主要因素,以菊糖得率为响应值利用Design Expert V8.0中心组合试验设计,建立菊糖提取得率的二次回归方程,得到优化组合条件。响应面法分析结果表明,当液固比为18∶1(m L·g^(- 1)),提取时间41min,提取温度86℃时,验证优化工艺得菊芋块茎中菊糖的最大提取得率40.56%,接近于模型预测值40.74%。在该工艺条件下,对菊糖进行粗制,并通过红外光谱仪对所提取的菊糖进行了结构分析。该法用于提取菊芋块茎中的菊糖,工艺简单、成本低,具有一定的应用价值。Single-factor tests and response surface method （RSM） were used to optimize the extraction process of Helianthus tuberosus L. inulin in tubers. In order to obtain the maximum of extraction yield of the inulin, three main factors including liquid-solid ratio, extraction times and extraction temperature were selected with the Design Expert V8.0 design, and a quadric regression equation for predicting the yield of inulin was established. The results showed that the optimum extraction conditions were as follows： liquid-solid ratio of 18：1 （mL ~ g-l）, extraction time of 41 rain and extraction temperature of 85~C. Under the conditions, the optimum extracting yield of inulin was 40.56%, which is well matched with the predictive extracting yield of 40.74%. Under the process, we carried out the rough inulin and analyzed extraction of the inulin by infrared spectrum with the simple and low cost process.

关 键 词：菊芋 菊糖 响应面法 提取工艺 

分 类 号：TS244.1[轻工技术与工程—制糖工程]