AXL对EGFR突变非小细胞肺癌细胞增殖的影响  

Effect of AXL on the Cell Proliferation of Non-small Cell Lung Cancer with EGFR Mutation

作　　者：田亚琼[1,2] 杨杰[2] 苗立云[2] 王永生[2] 蔡后荣[2] 

机构地区：[1]南京大学医学院,江苏南京210008 [2]南京大学医学院附属鼓楼医院,江苏南京210008

出　　处：《肿瘤学杂志》2016年第6期478-481,共4页Journal of Chinese Oncology

基　　金：国家自然科学基金项目(81301882);南京大学苗圃项目(2062014118)

摘　　要：[目的]探讨AXL的表达能否影响含EGFR突变肺癌细胞的生长增殖能力。[方法]研究使用了含EGFR第19外显子突变的PC9细胞株及具有更强增殖活性的耐吉非替尼的PC9细胞(PC9GR),使用基因工具使PC9细胞AXL过表达,及敲除AXL在PC9GR的表达,然后分别采用MTT试验检测转染后细胞的活力,比较实验组和对照组细胞对吉非替尼的敏感性,并计算IC50,评估细胞的增殖能力,另外选取69例EGFR突变肺癌患者的组织标本进行免疫组织化学染色,分析AXL表达状态与患者各项临床特征之间的关系。[结果]PC9细胞AXL过表达后,细胞增殖能力明显增强,敲除PC9GR细胞AXL表达后,耐药细胞的增殖能力减弱,免疫组织化学染色结果发现AXL阳性患者的肿瘤分化程度较阴性患者低,肿块大小明显大于AXL阴性患者。[结论]AXL与PC9肺癌细胞的增殖能力相关,同样也与临床患者的肿瘤分化程度和肿瘤大小相关,提示AXL可能成为一个潜在的肺癌治疗靶点。[Objective] To investigate the effect of AXL on the cell proliferation of non-small cell lung cancer with EGFR mutation. [Methods] We used lentivirus as an expression vector to force AXL overexpressed in PC9 cell lines which harbor EGFR 19 Del mutation. Meanwhile we silenced AXL in PC9 cell lines which had acquired the resistance to gefitinib and possessed higher proliferation capability than PC9,abbreviated to PC9 GR. MTT assays were used to detect the viability of these cells then the cell vitality curve was pictured and IC50 was calculated. At the other hand,we enrolled 69 patients who harbored EGFR mutation to conduct immunohistochemical staining. The relevance between AXL expression and clinical characteristics of these patients were analyzed. [Results] The viability of PC9 and PC9 GR was enhanced and suppressed after the upregulation and inhibition of AXL,respectively. The staining data suggested that AXL status was related to tumor differentiation and tumor size. [Conclusion] AXL may play an important role in tumor occurrence and development and may be a novel target to NSCLC treatment.

关 键 词：癌 非小细胞肺 EGFR AXL 

分 类 号：R734.2[医药卫生—肿瘤]