超声-酶法提取两面针中白屈菜红碱及其6-烷氧基衍生物的工艺优选  

Optimization of Ultrasonic Wave-enzymes Extraction Technology for Chelerythrine and Its 6-Alkoxy Derivatives from Zanthoxyli Radix

作　　者：陆世惠[1] 林瑶[1] 陈冉[1] 卢红梅[1] 

机构地区：[1]右江民族医学院药学院,科学实验中心,广西百色533000

出　　处：《中国实验方剂学杂志》2016年第13期23-26,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西自然科学基金项目(2014GXNSFBA118185)

摘　　要：目的:优化超声-酶法提取两面针中白屈菜红碱及其6-烷氧基衍生物的工艺条件。方法:采用HPLC测定白屈菜红碱及其6-烷氧基衍生物提取率,流动相乙腈-水-磷酸-三乙胺(25∶75∶0.2∶0.25),检测波长284 nm。通过单因素试验考察溶剂加酸和酶解预处理对有效成分提取率的影响,利用正交试验优化提取次数、超声功率和溶剂用量,通过动态过程优化超声时间。结果:最佳工艺条件为复合酶预处理后,加40%乙醇(含盐酸0.5%)于250 W超声提取3次,每次的溶剂用量分别为6,3,3倍,提取时间依次为18,15,12 min。白屈菜红碱及其6-烷氧基衍生物提取率90.5%。结论:该工艺经济、高效、节能、省时,可为开发利用两面针中白屈菜红碱等成分提供实验基础。Objective： To optimize technological conditions of ultrasonic wave-enzymes extraction for chelerythrine and its 6-alkoxy derivatives from Zanthoxyli Radix. Method： HPLC was employed to determine contents of chelerythrine and its 6-alkoxy derivatives with mobile phase of acetonitrile-water-phosphoric acidtriethylamine（ 25∶75∶0. 2∶0. 25） and detection wavelength at 284 nm. Orthogonal test and single factor tests were adopted to optimize extraction process of chelerythrine and its 6-alkoxy derivatives from Zanthoxyli Radix. Result：The optimal process was as follows： pretreated Zanthoxyli Radix powder with cellulase（ 1 ∶ 250） and pectinase（ 1∶250） for 30 min in 3. 7 times volume of acetic acid-sodium acetate buffer（ p H 5） at ambient temperature（ ≥30℃）,and then extracted 3 times by ultrasonic-wave（ 250 W） with 40% ethanol（ containing 0. 5% hydrochloric acid） as solvent,extracted 18 min with 6 times solvent at the first time,extracted 15 min with 3 times solvent at the second time,extracted 12 min with 3 times solvent at the third time. The extraction rate of chelerythrine and its 6-alkoxy derivatives was 90. 5%. Conclusion： This optimized process can improve economical benefit and efficiency,save energy and time,which provides experimental base for industrial production of chelerythrine from Zanthoxyli Radix.

关 键 词：两面针 白屈菜红碱 乙氧基白屈菜红碱 超声法 酶解法 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]