降胆固醇植物乳杆菌I4增菌培养基响应面优化  

Optimization of lowering cholesterol Lactobacillus Plantarum I4 enrichment mediumusing response surface method

作　　者：朱奇奇 蒲博[1] 张驰翔 王周[1] 邹奕昊 焦士蓉[1] 

机构地区：[1]西华大学食品与生物工程学院,成都610039 [2]惠氏制药有限公司,苏州215000

出　　处：《中国食品添加剂》2016年第6期87-95,共9页China Food Additives

基　　金：西华大学省级重点试验室开放研究基金项目(szjj2014-009);西华大学研究生创新基金资助(ycjj2015025);教育部春晖计划(12205543)

摘　　要：采用响应面法对降胆固醇植物乳杆菌I4增菌培养基进行优化研究。通过Plackett-Burman试验设计筛选出影响菌体浓度的主要因素为蔗糖、酵母提取物和乙酸钠。经响应面优化后,得到蔗糖、酵母提取物和乙酸钠的最佳浓度分别为2.05%、1.54%、0.31%。优化后的增菌培养基中,菌体密度OD_(620)可达2.458,比MRS培养基OD_(620)值提高了23.7%,菌落单位提高了3.91倍,通过对I4菌发酵剂降胆固醇能力的测试,得出胆固醇降解率为36.2±1.75(%,n=3)。Response surface methodology was used in lowering cholesterol Lactobacillus I4 enrichment medium optimization studies. Plackett-Burman experimental design was applied in screening the main factors affecting cell concentration of sucrose,yeast extract and sodium acetate. After the response surface to obtain a sugar,yeast extract. The optimum concentration of sodium acetate was 2.05%,1.54%,0.31%,the optimized enrichment medium cell density was up to 2.458 OD_（620）. The OD_（620） value improved 23.7% than in MRS medium,colony unit sincreased 3.91 fold. The test of the cholesterol-lowering ability of the formula indicates a degrading rate of 36.2±1.75（%,n=3）.

关 键 词：响应面法 Plackett-Burman试验设计 增菌培养基 菌体密度 

分 类 号：Q93-335[生物学—微生物学]