响应面优化芦笋黄酮的酶法辅助提取工艺  

Response surface optimization of extraction technology of flavonoids from asparagus waste assisted with enzymatic method

作　　者：李琴[1] 罗飞[1] 吴文湧 彭晓波[1] 杨江科[1] 

机构地区：[1]武汉轻工大学生物与制药工程学院,湖北武汉430023

出　　处：《武汉轻工大学学报》2016年第2期109-113,共5页Journal of Wuhan Polytechnic University

摘　　要：采用响应面法对果胶酶酶解和乙醇抽提的方式分步从芦笋皮中提取黄酮类化合物的工艺参数进行了优化。通过SAS 9.0软件分析,确定了最佳工艺参数:在液料比42.2∶1,时间87 min,温度75.9℃的情况下,对酶解后的芦笋废弃物中黄酮进行乙醇萃取,黄酮提取量约为7.28±0.21 mg/g,与预测值(7.37 mg/g)非常相近,拟合系数R2(96.67%)显示该回归模型可准确地预测果胶酶预处理之后的芦笋废弃物中黄酮的乙醇提取效率。Experiments were carried to optimize the ethanol extraction parameters of flavonoids from asparagus peel which was hydrolyzed by pectinase using response surface methodology in the study. All the data were analyzed by SAS 9.0 software and the optimized conditions were obtained as follows： solid-liquid ratio 42.2： 1,87 min,75.9 degrees.Under the optimum conditions,the quantity of flavonoids extracted was up to 7.28 mg / g,which was close to the predicted value（ 7.37 mg / g）. The model had a satisfactory coefficient of R2（ 96.67%） and verified experimentally. The results showed that response surface methodology was a useful method to optimize the ethanol extraction parameters of flavonoids from asparagus peel which was hydrolyzed by pectinase.

关 键 词：芦笋皮 响应面法 果胶酶 黄酮 

分 类 号：TS201.1[轻工技术与工程—食品科学]