三孢布拉霉菌发酵番茄红素提取工艺的优化  

Optimization of Extraction of Lycopene from Fermentation by Blakeslea trispora

作　　者：庞昆云 吴嘉琦[1] 王泽建[1] 储炬[1] 庄英萍[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心(上海),上海200237

出　　处：《华东理工大学学报（自然科学版）》2016年第3期343-350,共8页Journal of East China University of Science and Technology

基　　金：中荷国际项目(2013DFG32630)

摘　　要：番茄红素作为一种类胡萝卜素,具有提高免疫力、延缓衰老等多种功能。利用发酵法生产番茄红素是满足市场需求的重要途径。为了更好地提升番茄红素的提取收率,降低发酵过程中各种杂质的含量。研究了不同的溶媒及处理方法对湿菌体进行直接高效萃取的新方法。将番茄红素发酵液经过抽滤、洗涤处理得到湿菌丝体,采用有机溶剂和超声波破碎结合的处理方法,考察了菌丝体状态、皂化条件、脱水剂、提取溶剂、菌丝量与提取溶剂的料液比例、提取时间和提取温度对番茄红素提取率的影响。实验结果表明,利用湿菌体直接提取番茄红素的方法是可行的,并确定了湿菌体直接提取的最优条件:用0.6mol/L NaOH溶液皂化,乙醇脱水处理10min,乙酸乙酯作为提取剂,菌丝体质量(g)与提取溶剂体积(mL)之比为1∶75,在45℃超声处理20min,可获得最高的番茄红素提取率。As a kind of carotenoids, lycopene has the functions such as enhancing immunity,anti-aging, which is mainly produced by microorganism fermentation to meet the market demand. To enhance the extract yield, and reduce complex impurity components in the fermentation broth, different solvents and methods are used to process wet mycelium in this paper. By the combination of organic solvents extraction and ultrasonication, the extract effects of mycelium state, saponification conditions, dehydrating agent, extraction solvents, the ratio of mycelium biomass and extraction solvent, extraction time and extraction temperature were tested and all the samples used had been filtered and washed before. The results showed that it was feasible to extract lycopene directly from wet mycelium,and the optimum conditions were listed below：the saponification agent of 0. 6 mol /L NaOH, ethanol dehydrating time of 10 min, the ratio of mycelium biomass （g） and extraction solvent volume （mL） of 1 ： 75, ultrasonic processing time of 20 min at 45℃ with ethyl acetate as extraction agent.

关 键 词：三孢布拉霉菌 番茄红素 有机溶剂 提取 

分 类 号：Q815[生物学—生物工程]