Effect of periocular injection of celecoxib and propranolol on ocular level of vascular endothelial growth factor in a diabetic mouse model  

作　　者：Samar Nassiri Gholamreza Houshmand Mostafa Feghhi Alireza Kheirollah Mohammad Bahadoram Nariman Nassiri 

机构地区：[1]Department of Ophthalmology,Infectious Ophthalmic Research Center,School of Medicine,Ahvaz Jundishapur University of Medical Sciences,Ahvaz 61357-15794,Khuzestan,Iran [2]Department of Pharmacology,School of Pharmacy,Ahvaz Jundishapour University of Medical Sciences,Ahvaz 61357-15794,Khuzestan,Iran [3]Department of Biochemistry,Cellular&Molecular Research Center,Ahvaz Jundishapur University of Medical Sciences,Ahvaz 61357-15794,Khuzestan,Iran [4]Medical Student Research Committee and Social Determinant of Health Research Center,Ahvaz Jundishapur University of Medical Sciences,Ahvaz 61357-15794,Khuzestan,Iran [5]Jules Stein Eye Institute,David Geffen School of Medicine,University of California at Los Angeles,Los Angeles 90095,California,USA

出　　处：《International Journal of Ophthalmology(English edition)》2016年第6期821-824,共4页国际眼科杂志（英文版）

基　　金：Supported by the Ahvaz Jundishapur University of Medical Sciences(No.IORC-9203)

摘　　要：AIM： To investigate the effects of periocular injection of propranolol and celecoxib on ocular levels of vascular endothelial growth factor （VEGF） in a diabetic mouse model. METHODS： Forty 4-6wk BALB-C male mice weighing 20-25 g were used. The study groups included： nondiabetic control （group 1）, diabetic control （group 2）, diabetic propranolol （group 3）, and diabetic celecoxib （group 4）. After induction of type 1 diabetes by streptozotocin, propranolol （10 μg） and celecoxib （200 μg dissolved in carboxymethylcellulose 0.5%） were injected periocularly. The ocular level of VEGF was measured in all the study groups using enzyme-linked immuno sorbent assay （ELISA） method. RESULTS： Ocular VEGF level was significantly increased （1.25 fold） in the diabetic control group when compared to the non-diabetic group one week after induction with streptozotocin （P=0.002）. Both periocular propranolol and celecoxib significantly reduced ocular VEGF levels （P=0.047 and P〈0.001, respectively）. The effect was more pronounced with celecoxib, CONCLUSION： The periocular administration of propranolol and celecoxib can significantly reduce ocular VEGF levels in a diabetic mouse model.AIM： To investigate the effects of periocular injection of propranolol and celecoxib on ocular levels of vascular endothelial growth factor （VEGF） in a diabetic mouse model. METHODS： Forty 4-6wk BALB-C male mice weighing 20-25 g were used. The study groups included： nondiabetic control （group 1）, diabetic control （group 2）, diabetic propranolol （group 3）, and diabetic celecoxib （group 4）. After induction of type 1 diabetes by streptozotocin, propranolol （10 μg） and celecoxib （200 μg dissolved in carboxymethylcellulose 0.5%） were injected periocularly. The ocular level of VEGF was measured in all the study groups using enzyme-linked immuno sorbent assay （ELISA） method. RESULTS： Ocular VEGF level was significantly increased （1.25 fold） in the diabetic control group when compared to the non-diabetic group one week after induction with streptozotocin （P=0.002）. Both periocular propranolol and celecoxib significantly reduced ocular VEGF levels （P=0.047 and P〈0.001, respectively）. The effect was more pronounced with celecoxib, CONCLUSION： The periocular administration of propranolol and celecoxib can significantly reduce ocular VEGF levels in a diabetic mouse model.

关 键 词：diabetic retinopathy CELECOXIB PROPRANOLOL vascular endothelial growth factor NEOVASCULARIZATION diabetic mouse model 

分 类 号：R77[医药卫生—眼科]