桑色素对视网膜母细胞瘤生长和凋亡的影响  

Effect of morin on proliferation and apoptosis of retinoblastoma cells

作　　者：白淑玮[1] 李凤至[1] 姚倩[1] 

机构地区：[1]陕西省西安市第四医院眼科,710004

出　　处：《眼科》2016年第3期171-174,共4页Ophthalmology in China

基　　金：国家自然科学基金项目(81302198)

摘　　要：目的探讨桑色素对人视网膜母细胞瘤细胞增生抑制及诱导凋亡的作用。设计实验研究。研究对象人视网膜母细胞瘤细胞Y79细胞。方法不同浓度(0、50、100、150、250μM)桑色素对人视网膜母细胞瘤细胞Y79细胞作用24、48、72小时后,CCK8法检测肿瘤细胞生存率的变化;细胞周期检查法检测肿瘤细胞周期的变化;用TUNEL法检测肿瘤细胞凋亡的变化。主要指标细胞生存率、细胞周期分布、细胞凋亡率。结果桑色素浓度为150、250μM,与桑色素浓度为0、50、100μM作用于Y79细胞相比,检测时间分别在48、72小时时的细胞生存率显著下降(P<0.05),细胞周期G0-G1期显著增多(P<0.05),S期显著下降(P<0.05),细胞凋亡显著增多(P<0.05)。较高浓度的桑色素,随着作用时间的延长,引起Y79细胞生存率逐渐下降,细胞凋亡率逐渐增多,具有时间依赖性和剂量依赖性。结论桑色素能够抑制视网膜母细胞瘤增生并促进其凋亡,提示桑色素有望成为治疗视网膜母细胞瘤的有效药物。Objective To examine the effect of morin on the proliferation and apoptosis of cultured retinoblastoma cells. Design Experimental study. Participants The retinoblastoma cell lines Y79. Methods The retinoblastoma cell lines Y79 were treated with morin in increasing concentrations（0, 50, 100, 150, 250 μM respectively） for 24, 48, and 72 hours and then the cell proliferation rate was measured. Cell cycle was analyzed by flow cytometry, and cell apoptosis rate was measured by TUNEL staining. Main Outcome Measures The percentage of cell survival, the distribution of cell cycle, the percentage of apoptotic cells to total cells. Results After the morin acting on the Y79 cells at the concentrations of 150 μM or 250 μM for 48, 72 h respectively, the percentages of tumor cell survival rate decreased significantly（P〈0.05） compared to those at the concentrations of 0, 50 or 100 μM. Correspondingly, the percentage of apoptotic cells to total cells was significantly（P〈0.05） higher in the Y79 cells treated by morin at the concentrations of 150 μM or250 μM than at the concentrations of 0, 50 or 100 μM. In a parallel manner, the cell cycle was significantly（P〈0.05） altered in the Y79 cells treated by morin at the concentrations of 150 μM or 250 μM,with increased percentages of Y79 cells in the G0/G1 arrest and reduced percentages of Y79 cells in the S phase. The morin caused concentration-dependent and time-dependent decreasing in cell proliferation and increasing in cell apoptosis. Conclusion Mrion added to cultures of retinoblastoma cells led to a reduced tumor cell proliferation, and an increased tumor cell apoptosis, which suggests that morin may be a potent drug for the treatment of retinoblastoma.

关 键 词：桑色素 视网膜母细胞瘤 增生 凋亡 

分 类 号：R739.7[医药卫生—肿瘤]