气相色谱-质谱法定性定量测定糜子中的脂肪酸  

Identification and Quantitation of Fatty Acids in Panicum miliaceum L. by Gas Chromatography-Mass Spectrometry

作　　者：田翔[1] 乔治军[1] 田琴[1] 曹晓宁[1] 王君杰[1] 刘思辰[1] 

机构地区：[1]山西省农业科学院农作物品种资源研究所,农业部黄土高原作物基因与种质创制重点实验室,杂粮种质资源发掘与遗传改良山西省重点实验室,山西太原030031

出　　处：《食品科学》2016年第14期128-131,共4页Food Science

基　　金：山西省农业科学院攻关项目(ygg-1514);国家现代农业产业技术体系建设专项(CARS-07-12.5-A12)

摘　　要：采用气相色谱-质谱法对糜子中脂肪酸进行定性、定量分析。结果共鉴定出14种脂肪酸,相对含量为96.9%,其中不饱和脂肪酸8种(64.533%),主要有亚油酸(12.371%)、油酸(44.157%)、亚麻酸(1.173%)等。可见糜子中脂肪酸主要以不饱和脂肪酸为主,是优质脂肪酸。通过回归方程计算得出:糜子中棕榈酸、油酸、硬脂酸、亚油酸、亚麻酸的含量分别为100.261、165.309、23.402、52.181、17.516 mg/100 g。通过与其他杂粮进行比较,结果表明糜子和小米、高粱、小麦、燕麦、水稻一样均含有硬脂酸、油酸、亚油酸、亚麻酸和棕榈酸等,不饱和脂肪酸含量高,富含亚油酸和亚麻酸;本研究可为糜子品质的进一步开发和研究提供理论依据。Fourteen fatty acids in Panicum miliaceum L. were qualitatively identified and quantitatively determined using gas chromatography-mass spectrometry（GC-MS）. The total content of these fatty acids was determined to be 96.9%. Among the identified fatty acids were 8 unsaturated fatty acids（64.533%）, of which the predominant ones were linoleic acid（12.371%）, oleic acid（44.157%）, linolenic acid（1.173%）, etc. Obviously, the dominant fatty acids in Panicum miliaceum L. were unsaturated fatty acids, which are high quality fatty acids. The results calculated from the regression equation showed that the contents of palmitic acid, oleic acid, stearic acid, linoleic acid and linolenic acid in Panicum miliaceum L. were 100.261, 165.309, 23.402, 52.181 and 17.516 mg/100 g, respectively. As with other grains such as foxtail millet, sorghum, wheat, oat and rice, Panicum miliaceum L. contained stearic acid, oleic acid, linoleic acid, palmitic acid and linolenic acid, rich in unsaturated fatty acids, especially linoleic acid and linolenic acid.

关 键 词：糜子 脂肪酸 气相色谱-质谱法 

分 类 号：S515[农业科学—作物学]