大豆乳清废水中β-淀粉酶工业生产工艺的研究  

Industrial production research of β-amylase from soybean whey wastewater

作　　者：关艳艳[1] 牛延宁[1] 贾彩凤[1] 高红亮[1] 崔红亮 常忠义[1] 

机构地区：[1]华东师范大学生命科学学院,上海200241 [2]平顶山天晶植物蛋白有限公司,平顶山467000

出　　处：《食品科技》2016年第6期293-297,共5页Food Science and Technology

摘　　要：大豆乳清废水中含有较高含量的β-淀粉酶,分别用超滤和乙醇沉淀方法分离大豆乳清废水中的β-淀粉酶,并确定其最佳条件。选用截留分子量为20000 u的超滤膜,在跨膜压差(Δp)为0.25 MPa下,2级超滤9倍,然后先加入冰无水乙醇至乙醇体积分数为30%(v/v)沉淀以除去杂质,再加入冰无水乙醇使乙醇体积分数为70%(v/v)沉淀β-淀粉酶。沉淀用50 mmol/L、p H6.0醋酸钠缓冲液复溶,复溶体积为超滤后体积的1/10,最后得到的β-淀粉酶酶液单位酶活为118600 U/m L,酶活得率为77.54%。应用超滤和乙醇沉淀相结合的方法,使得从大豆乳清废水中大规模地生产β-淀粉酶成为可能。Soybean wheywaste water containing high content of beta amylase. Respec tively using ultrafiltration and alcohol precipitation method in the separation of soybean whey waste water beta amylase, and to determine the best conditions. Experiment results showed that choosing the ultrafiltration membrane of 20000 intercept molecular weight, transmembrane pressure（Δp）=0.25 MPa, concentrating 9 times in ultrafiltration grade separation method, and then the wastewater was fractionated by the dropwise addition of 30%（v/v） of chilled absolute alcohol to remove the impurities. The supernatant was collected and then added the absolute alcohol until the final concentration was 70%. The precipitated proteins were resolved in 50 mmol/L acetate buffer（p H=6）, the dissolving volume is 1/10 of ultrafiltration volume. Finally we get the β-amylase enzyme liquid with enzyme activity of 118600 U/m L and 77.54% yield. Combing the application of ultrafiltration and alcohol sedimentation, we found combining the two methods and choosing the appropriate, we can effectively realize the industrialization production of β-amylase from soybean whey wastewater.

关 键 词：大豆乳清废水 Β-淀粉酶 工业生产工艺 

分 类 号：TS239[轻工技术与工程—粮食、油脂及植物蛋白工程]