奶粉中阴沟肠杆菌实时荧光PCR快速检测方法建立  

Rapid Detection of Enterobacter Cloacae in Powdered Milk by Real-Time PCR

作　　者：周慧平[1] 唐连飞[1] 蔡文杰[2] 谭建锡[1] 朱中武[1] 禹思宇[1] 

机构地区：[1]湖南出入境检验检疫局,湖南长沙410004 [2]湖南省畜牧兽医研究所

出　　处：《检验检疫学刊》2016年第3期10-13,共4页Journal of Inspection and Quarantine

基　　金：湖南省科技计划项目(2014SK3082)

摘　　要：为建立快速有效的阴沟肠杆菌检测方法,根据Genbank中的阴沟肠杆菌Amp C酶基因序列设计引物和探针,建立了奶粉中阴沟肠杆菌实时荧光PCR快速检测方法。结果显示,该方法只对阴沟肠杆菌Amp C酶基因呈阳性反应,而对其他常见非阳性菌株基因组DNA均呈阴性反应,检测限为20 CFU/m L,对人工污染的奶粉样品检测验证了方法的实用性。建立的实时荧光PCR方法特异性强,灵敏度高,可以快速、准确检测奶粉中污染的阴沟肠杆菌。The specific primers and probe for the real-time PCR according to the Enterobacter cloacae Amp C nucleotide sequence were designed and real-time PCR method was established to detect DNA of Enterobacter cloacae. The results showed that Enterobacter cloacae genes were positive by real-time PCR while other common non-positive strains genomic DNA was negative. Milk powder samples of artificial pollution test the practicality of the method is validated and the detection limit of Enterobacter cloacae were 20CFU/mL. The above results demonstrated that the method could be used for rapidly and accurately detect the Enterobacter cloacae contamination in powdered milk with high specificity and sensitivity.

关 键 词：阴沟肠杆菌 AMPC酶 实时荧光PCR 检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]