Box-Behnken设计-响应面法优化鹰嘴豆油提取工艺  

Optimization of Extraction Technology of Chickpea Oil by Box-Behnken Design and Response Surface Method

作　　者：程坤[1] 王威[1] 刘小红[1] 姚慧丽[1] 高琪[1] 刘洋[1] 高华[1] 

机构地区：[1]青岛大学药学院,山东青岛266021

出　　处：《青岛大学学报（工程技术版）》2016年第2期82-88,共7页Journal of Qingdao University(Engineering & Technology Edition)

摘　　要：针对鹰嘴豆油6#溶剂提取工艺,本文在单因素实验的基础上,对鹰嘴豆油6#溶剂提取工艺进行研究。以提取温度、提取时间和液料比为自变量,以提取量和过氧化值为响应值建立数学模型,并根据Box-Behnken设计原理,建立二次多项式回归方程预测模型,以达到高度显著水平,而失拟项不显著;在提取量响应面分析中,一次项、交互项和二次项中的提取温度和液料比极为显著;在过氧化值响应面分析中,一次项和二次项中的提取时间极为显著。为检验响应面法预测的可靠性,在最佳平衡提取工艺条件,即在提取温度为62.1℃,提取时间为2.13h,料液比为6.9∶1,鹰嘴豆油提取量为3.477 6g/100g,过氧化值为1.54mmol/kg的条件下,进行三批放大工艺验证实验。实验结果表明,鹰嘴豆油提取量分别为3.464 7,3.471 9,3.467 3g/100g,过氧化值分别为1.57,1.52,1.54mmol/kg,该结果与理论值基本吻合,说明Box-Behnken设计-响应面法优化的鹰嘴豆油提取工艺可行。该研究为鹰嘴豆油的开发利用提供了理伦参考。To optimize the technology of chickpea oil extracted with 6 # solvent by Box-Behnken design and response surface method, on the basis of single factor tests, a three-level and three-variable Box-Behnken design was applied for the optimization of the processing parameters. The three independent variables were extraction temperature, time and liquid to solid ratio. The empirical second-order polynomial models for extraction volume and peroxide value of chickpea oil were established with extremely statistical signifi- cance, but the lack of fits was statistical insignificance. In the response-surface analysis, linear term, quadratic term, and interaction item of extraction temperature and liquid ratio for extraction volume, and linear term and quadratic term of extraction time for peroxide value were extremely significant model terms. Simultaneously taking into account the extraction volume and peroxide value, the optimized extrac- tion conditions were extraction temperature of 62.1 ℃, extraction time 2.13 h, and liquid to solid ratio 6.9 ： 1. The extraction volume of 3. 477 6 g/100 g and peroxide value of 1.54 mmol/kg were obtained from experiments. The result of the validation tests basieally tallied with the theoretically analyses,which could provide a reference for development and utilization of chickpea oil.

关 键 词：鹰嘴豆 BOX-BEHNKEN设计 响应面法 植物油 提取工艺 

分 类 号：TS224[轻工技术与工程—粮食、油脂及植物蛋白工程]