青皮竹中酚酸类和碳苷黄酮类HPLC分析方法的建立  

HPLC method development for analysis of phenolic acids and flavone C- glycosides in Bambusa textiles McClure

作　　者：马娜[1] 刘孟华[2] 丁林伟 付卫明[1,2] 何敬愉[1] 

机构地区：[1]广州中国科学院先进技术研究所生物工程研究中心,广东广州511458 [2]南方医科大学药学院,广东广州510515 [3]广州海关化验中心,广东广州510623

出　　处：《食品工业科技》2016年第14期90-92,97,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(81503376);广州市科技计划项目(2014J4100045)

摘　　要：建立了一种高效液相色谱(HPLC)法对青皮竹提取物中的三种酚酸和四种碳苷黄酮进行定量分析的方法,并进行了方法学考察。得到最佳色谱条件为:C18柱(4.6 mm×150 mm,5μm),以甲醇:0.1%磷酸水为流动相,流速为1.0 m L/min,UV检测波长254 nm,洗脱程序为0~10 min,10%~22%甲醇;10~30 min,22%~35%甲醇;30~40 min,35%~60%甲醇。结果表明,对羟基苯甲酸、香草酸、对香豆酸、荭草苷、异荭草苷、牡荆素、异牡荆苷的线性范围分别在0.08~100、0.04~100、0.25~50、0.24~60、0.22~56、0.06~56、0.12~60μg/m L之间,R^2大于0.9996,加标回收率在96%~103%之间,RSD小于3%,日内精密度和日间精密度都小于3.48%,该方法灵敏度高,重复性好,方法学验证符合要求。此方法可用于青皮竹中三种酚酸和四种碳苷黄酮的含量检测。An analytical method based on high performance liquid chromatography（ HPLC） was developed for quantification of three phenolic acids and four flavone C- glycosides in Bambusa textiles Mc Clure,and was also validated.The optimum chromatographic conditions were determined using a C18column（ 4.6 mm × 150 mm,5 μm）at the detection wavelength of 254 nm and a flow rate of 1.0 m L / min.Mobile phase was a binary eluent of methanol（ A） and 0.1% aqueous phosphoric acid（ v /v）（ B） with gradient conditions as follows： 0 ~ 10 min,10% ~ 22% A,10~30 min,22% ~35% A,30 ~ 40 min,35% ~ 60% A. As a result,p- hydroxybenzoic acid,vanillic acid,p- coumaric acid,orientin,isoorientin,vitexin and isovitexin had good linearity（ R^2≥0.9996） within range of 0.08~100,0.04~100,0.25~50,0.24~60,0.22~56,0.06 ~ 56 and 0.12 ~ 60 μg / m L,respectively. The recoveries of the analytes were between96% to 103%（ RSD 3%）,and the RSD of intraday and interday precisions were all less than 3.48%. The method was of high sensitivity,good reproducibility,and meets the methodological requirements. The method could apply to determine the contents of three phenolic acids and four flavone C- glycosides in B.textiles.

关 键 词：青皮竹 酚酸 碳苷黄酮 HPLC 

分 类 号：TS201.2[轻工技术与工程—食品科学]