液氨预处理工艺对五节芒酶解糖化效果的影响  

Effects of Liquid Ammonia Treatment on Enzymatic Hydrolysis to Fermentable Sugars of Miscanthus floridulus

作　　者：彭何欢[1] 邵千钧[1,2] Farman Ali Chandio 赵超[1] 李彬[1] 马中青[1] 

机构地区：[1]浙江农林大学工程学院,杭州311300 [2]宁波大学机械工程与力学学院,宁波315211 [3]信德农业大学农业工程学院,登多贾姆70060

出　　处：《农业机械学报》2016年第7期196-201,共6页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家自然科学基金项目(31500491);浙江农林大学生物质利用研究中心预研项目(2013SWZ03)

摘　　要：为提高五节芒可发酵单糖的产量,采用液氨对其进行预处理。选用预处理温度T、五节芒含水率M、驻留时间t和液氨用量比k为变量因素,在试验范围内T（70～170℃）、M（20%～200%）、t（5～30 min）、k（0.5～5.0）通过对各因素的不同取值,分析上述因素对五节芒酶解效果的影响。结果表明：在T=130℃、M=80%、t=10 min、k=2.0的条件下预处理,五节芒72 h的葡聚糖和木聚糖酶解率分别为72.1%和82.5%,即100 g干基原材料可获得可发酵单糖55.8 g,是未经预处理样品酶解率的3.65倍。因此,液氨预处理能够有效促进五节芒的酶解单糖转化率。To effectively improve the enzymatic hydrolysis rate of carbohydrates in lignocelluloses,the liquid ammonia treatment（ LAT） was employed as pretreatment for Miscanthus floridulus. The pretreatment temperature T,Miscanthus floridulus moisture M,residence time t and the ratio of ammonia to biomass（ dry basis） k were chosen as variable factors. In the experimental ranges of T（ 70 ~170 ℃）,M（ 20% ~ 200%）,t（ 5 ~ 30 min） and k（ 0. 5 ~ 5. 0）,the influences of different variable factors on enzymatic hydrolysis of Miscanthus floridulus were investigated. The chemical composition analysis showed that the chemical constitution of Miscanthus floridulus was close to Saccharum arundinaceum and Miscanthus sinensis,and it could be a prior species of plant energy. The pretreatment methods and effects of these biomasses could be used as reference for each other. The parameter optimization results show that the optimal LAT pretreatment conditions for Miscanthus floridulus were130℃,80% moisture content,10 min residence time and 2. 0 ammonia to biomass ratio. Enzymatic hydrolysis using cocktail enzymes including cellulase, β-glucosidase and xylanase for 72 h after pretreatment under optimal conditions,the glucan and xylan hydrolysis rates of LAT-treated Miscanthus floridulus achieved 72. 1% and 82. 5%,which were 3. 3-fold and 4. 3-fold than those of untreated biomass respectively. The total fermentable monosaccharides after pretreatment under optimal conditions following enzymatic hydrolysis was 55. 8 g per 100 g of dry raw material. Compared with untreated substrates,it was about 3. 65-fold in sugar yield for LAT-treated substrates. As a result,the LAT process significantly reduced the recalcitrance of Miscanthus floridulus and facilitated biomass conversion to fermentable sugars.

关 键 词：五节芒 预处理 液氨 酶解 

分 类 号：TK6[动力工程及工程热物理—生物能] S216[农业科学—农业机械化工程]