Mdr_1反义寡核苷酸联合磁性载药微球逆转胰腺癌耐药实验研究  

Reversal of Multidrug Resistance Antisense Oligodeoxynucletedes and PEG-5-FU-MAMS Towards Human Pancreatic Cell Line

作　　者：赵会庚[1] 权广前[1] 潘耀振[1] 尹家俊[1] 

机构地区：[1]大连大学附属中山医院,116001

出　　处：《实用癌症杂志》2016年第7期1045-1049,共5页The Practical Journal of Cancer

基　　金：辽宁省自然科学基金项目(编号:201102006)

摘　　要：目的探讨辐射促细胞转染的多药耐药(multidrug resistance,MDR)基因Mdr1反义寡核苷酸(ASON)逆转肿瘤细胞SW1990/Fu的耐药效果。方法应用RT-PCR方法和流式细胞仪,检测2种不同的转染方法对SW1990/Fu细胞的Mdr1-mRNA及其表达产物P-糖蛋白(P-gp)的调控情况。结果反转录RT-PCR和流式细胞仪的结果显示,联合辐射的阳性脂质体介导ASON组的SW1990/Fu细胞Mdr1-mRNA的表达水平及细胞膜糖蛋白(P-gp)的阳性率均明显低于反义寡核苷酸(ASON)组(P<0.01)。结论辐射促转染的Mdr1ASON联合磁性载药微球对肿瘤细胞具有较好的耐药逆转作用。Objective To investigate the reversal the multidrug resisitance（ MDR） by MDR gene mdrl antisense oligodeoxynucletdes（ ASON） and Polyethylene Glycol modified 5-Fluorouracil magnetic albumin miscrospheres（ PEG-5-FU-MAMS） in the human pancreatic cancer cell line in SW1990 / Fu. Methods The expression of Mdr1 at mRNA level was examined by RTPCR. Flow cytometry was used to demonstrated the positive rate the Mdr1 gene product P-glucopretein（ P-gp） in lipo-ASON treated cells with 2 different methods. Results The inhibitory efficiency of lipo-ASON and ionizing radiation plus Polye-Thylene Glycol modified 5-Fluorouracil magnetic albumin miscrospheres（ PEG-5-FU-MAMS） was higher than that without ionizing radiation（ P〈0. 01）. The IC50,The Mdr1 RNA expression level,and P-gp positive rate of irradiated sw1990 / Fu cells were all significantly lower than those of unirradiated cells（ P〈0. 05）. Conclusion The reversal effect of Mdr1 ASON and ionizing radiation plus Polyethy-lene Glycol modified 5-Fluorouracil magnetic albumin miscrospheres（ PEG-5-FU-MAMS） is stronger on resistant cell than that of Mdr1 ASON and Polyethylene Glycol modified 5-Fluorouracil magnetic albumin miscrospheres（ PEG-5-FU-MAMS） alone.

关 键 词：电离辐射 反义寡核苷酸 转染 多药耐 PEG-5-FU-MAMS 

分 类 号：R735.9[医药卫生—肿瘤]