芦苇厌氧联产氢气-甲烷过程中古菌群落特征解析  

Archaea community structure succession in anaerobic fermentation of hydrogen and methane coproduction system

作　　者：贾璇[1] 任连海[1] 席北斗[2] 祝超伟[2] 李鸣晓[2] 赵由才[3] 

机构地区：[1]北京工商大学食品学院环境科学与工程系,北京100048 [2]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [3]同济大学污染控制与资源化研究国家重点实验室,上海200092

出　　处：《环境污染与防治》2016年第7期5-10,共6页Environmental Pollution & Control

基　　金：国家"十二五"科技支撑计划课题(No.2014BAL02B05)

摘　　要：采用厌氧联产氢气-甲烷工艺研究芦苇能源化利用潜力,解析联产过程中古菌群落特征和演替规律。产气情况表明,芦苇经纤维素酶预处理后累积产气量和氢气、甲烷产量显著提高。聚合酶链式反应—变性梯度凝胶电泳(PCR—DGGE)分析可知,厌氧联产过程中古菌群落结构发生了规律性变化,产甲烷阶段古菌微生物多样性显著高于产氢阶段,产氢阶段古菌群落结构相似性较高,随着产甲烷的进行古菌群落相似性降低,微生物多样性提高。甲烷囊菌属(Methanoculleus)、产甲烷古菌(Methanogenic archaeon)、甲烷杆菌属(Methanobacterium)和uncultured Methanosarcinales archaeon是芦苇厌氧联产过程的优势微生物,其中产氢阶段的优势菌为uncultured Methanosarcinales archaeon,能够利用乙酸途径产甲烷;产甲烷高峰期,古菌群落丰富,Methanoculleus bourgensis是产甲烷阶段优势菌。研究结果为秸秆厌氧联产工艺的生物强化与稳定运行提供了重要的微生物学依据。The coproduction of hydrogen and methane from aquatic plants has been considered a promising method for practical applications. The archaea community succession was investigated using PCR amplification of 16S rDNA genes couple with denaturing gradient gel electrophoresis analysis （PCR-DGGE） during reed straw anaerobic fermentation coproduction. The results showed that the kind and number of archaea in methanogenic stage was dra- matically higher than that of the hydrogenogenic stage. In hydrogenogenic stage,the relatively was higher than the methanogenic stage. The sequences of 16S rDNA DGGE predominant bands fragments were determined by compari- son with NCBI database. The majority of the sequences obtained was affiliated with Methanoculleus,Methanogenic archaeon ,Methanobacterium and uncultured Methanosarcinales archaeon. In hydrogenogenic stage, the dominant ar- chaea was uncultured Methanosarcinales archaeon ,which could be produced methane by acetate metabolism pathway. In methanogenic stage, the dominant archaea was Methanoculleus bourgensis, which could be produced methane by one carbon metabolism. The result indicated that dominant archaea could play an important role in reed straw utilization process and provide the evidence to coproduction system stability and efficiently.

关 键 词：芦苇 秸秆 氢气-甲烷联产 古菌 群落演替 

分 类 号：TQ920[轻工技术与工程—发酵工程] TQ116.2TQ221.11