异养小球藻发酵与树脂调节pH过程耦合工艺  

Coupling technology of heterotrophic Chlorella sp. fermentation and resin adjusting p H process

作　　者：毕生雷 张成明[2,3] 郑世文[1] 金洪波[1] 吴娟[1] 鲁龙[1] 张鹏飞[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000 [2]清华大学核能与新能源技术研究院,北京100084 [3]北京市生物燃料工程技术研究中心,北京100084

出　　处：《中国酿造》2016年第7期55-59,共5页China Brewing

基　　金："十二五"农村领域国家科技计划(2011BAD14B05)

摘　　要：为了解除发酵产酸对异养小球藻正常生长的抑制,提出了异养小球藻发酵与树脂调节p H过程耦合工艺。考察了添加树脂种类、添加树脂时间、树脂添加量对发酵过程的影响,结果发现,在发酵72 h添加8 g/L的凝胶树脂Lx-300C能够调节p H维持在中性,细胞数达到7.5×108个/m L,与空白组持平,发酵结束后发酵液干质量比空白组高5.59%。树脂能够反复使用4次。In order to remove the fermentation acid on the inhibition of normal growth of heterotrophic Chlorella sp., the coupling technology of het- erotrophic ehlorella fermentation and resin adjusting pH process was put forward. The effects of addition of resin varieties, adding time and adding amount on the fermentation process were investigated. It was found that adding the resin Lx-300C by 8 g/L could regulate the pH in neutral, the num- ber of cells reached 7.5 ×108 cell/ml, which was the same as that of the blank group. After fermentation, the weight of the fermentation broth was 5.59% higher than that of the blank group, the resin can be used repeatedly for four times.

关 键 词：异养小球藻 树脂 耦合 

分 类 号：TQ320.64[化学工程—合成树脂塑料工业]