乳铁蛋白在DMH-DSS诱导大鼠大肠癌中的抑制作用  

Inhibition of DMH-DSS-induced colorectal cancer by lactoferrin in rats

作　　者：金晟剑 左晓旭[1] 李皓[1] 朱袭嘉[1] 王海鹏[1] 

机构地区：[1]桂林医学院第二附属医院胃肠外科,广西桂林541100

出　　处：《华夏医学》2016年第3期18-21,共4页Acta Medicinae Sinica

摘　　要：目的:探讨乳铁蛋白(LF)在DMH-DSS(1,2-二甲基肼-葡聚糖硫酸钠)诱发大鼠大肠癌中的作用。方法:Wistar大鼠随机分为3组:对照(水)组12只,500 mg·kg^(-1)·d^(-1)LF组(LF低剂量组)12只和1000 mg·kg^(-1)·d^(-1)LF组(LF高剂量组)12只。所有实验大鼠在饮用1%DSS 1周后,皮下注射DMH(20 mg/kg),每周1次,连续8周。于注射DMH开始25周后处死所有大鼠。在显微镜下检测大肠腺瘤和腺癌发生的情况。结果:LF低剂量组和LF高剂量组与对照组大鼠相比,腺瘤和腺癌的数量明显减少.差异有统计学意义(P<0.05)。结论:LF对大鼠大肠癌的发生、发展具有抑制作用。Objective：We evaluated the anti-tumour effects of LF on 1, 2-dimethylhydrazine （DMH） / Dextran Sulphate Sodium（DSS）-induced model of carcinogenesis in Wistar rats. Methods： Wistar rats were randomized into three groups with 12 rats in each group： the control group（water）, 500mg/kg/day LF group （low-dose group） and 1000mg / kg / day LF group （high-dose group）. All experimental animals were supplemented with 1% DSS for one week. Afterwards the rats were injected with DMH （20 mg / kg body weight） once a week for 8 consecutive weeks. All rats were sacrificed after 25 weeks of DMH treatment and the number of colon adenomas and adenocarcinomas were examined. Results： Rats from the LF low-dose group and LF high-dose group harboured significantly fewer adenomas and adenocarcinomas than rats from the control group, which were of statistical significances （P〈 0.05）. Conclusion： LF can inhibit the occurrence and development of colorectal cancer in rats.

关 键 词：乳铁蛋白 大肠癌 DMH DSS 

分 类 号：R735.34[医药卫生—肿瘤]