高效液相色谱法测定玉米蛋白粉中游离氨基酸含量  

Determination of Free Amino Acids by High Performance Liquid Chromatography in Corn Gluten Meal

作　　者：刘跃芹[1] 吴延东[1] 赵雪松[1] 刘艳波[1] 吴杰[1] 闫寒[1] 

机构地区：[1]玉米深加工国家工程研究中心,长春130033

出　　处：《中国粮油学报》2016年第7期153-156,共4页Journal of the Chinese Cereals and Oils Association

基　　金：农业科技成果转化资金(2012GB2B100101)

摘　　要：建立高效液相色谱法同时测定玉米蛋白粉中17种游离氨基酸含量的方法。样品用盐酸溶液（0．1mol／L）提取，采用6-氨基喹啉-N-羟基琥珀酰亚胺基-氨基甲酸酯（AQC）为衍生剂衍生。应用No—va—PakTM C18柱，以10mmoL／L醋酸铵缓冲液、乙腈和超纯水为流动相进行梯度洗脱，柱温为37oC，检测波长为248nm，外标法定量。结果表明，17种游离氨基酸线性关系良好，其中16种游离氨基酸在0．0025～0．5μmoL／mL范围内具有良好的线性关系，胱氨酸浓度在0．0063～1．25μmol／mL范围内线性关系良好，相关系数在0．9991～0．9999之间，回收率为95．10％～105．21％，RSD为1．36％～4．39％。该方法灵敏度高、重现性好、操作简单，可用于检测玉米蛋白粉中游离氨基酸含量。High performance liquid chromatography was to be established along with a method for the determina- tion of 17 kinds of free amino acids in corn gluten meal by high performance liquid chromatography. The specimen was extracted with hydrochloric acid solution （0.1 mol/L） , and 6 -ami -noquinolyl -N -hydroxysuccinimidyl car- bamate （AQC） then was derivatized from the derivating agent. Gradient elution was achieved by using a Nova - Pak- TM C 18, and ammonium acetate buffer solution（ 10 mmol/L） , acetonitrile and deionized water as the mobile phase with the column temperature of 37 ℃, the detected wave length of 248 nm. The external standard method was used for quantification. Results show there was a good linear relationship for 17 free amino acids, of which a good linear relationship was identified in 16 free amino acids in the range of 0. 002 5 - 0.5 μmol/mL, while cystine in the range of 0. 006 3- 1.25 μmoL/ml. The correlation coefficient is in the range of 0. 999 1 -0. 999 9. Average recovery of 17 amino acids was 95.10% - 105.21% , and the RSD was in the range of 1.36%-4.39%. This method is highly sen- sitive, reproducible , simple and suitable for the determination of free amino acids in corn gluten meal.

关 键 词：高效液相色谱法 游离氨基酸 玉米蛋白粉 

分 类 号：O657.7[理学—分析化学]