昆虫内生真菌Chaetomium globosum ZWC5的分离鉴定及其抗癌活性代谢产物的研究  

Study on Isolation and Taxonomy Determination of Insect Derived Endophytic Fungus Chaetomium Globosum ZWC5 and Its Anticancer Active Metabolites

作　　者：杨会祥 邓园园[1] 李薇[1] 吕静南 徐婵[1] 马云瑞[1] 杨新洲[1] 林亲雄[1] 

机构地区：[1]中南民族大学药学院,中国武汉430074

出　　处：《湖南师范大学自然科学学报》2016年第4期17-22,95,共7页Journal of Natural Science of Hunan Normal University

基　　金：中南民族大学十二五"民族药学"国家级实验教学示范中心项目;国家级大学生创新训练项目(GXX14211)

摘　　要：为了研究中华稻蝗内生真菌ZWC5菌株及其活性代谢产物,通过菌株形态和其r DNA的ITS序列分析,鉴定其为球毛壳霉(Chaetomium globosum);采用MTT法和流式细胞技术测定其代谢产物的抗癌活性,结果表明其石油醚提取物对肝癌细胞HepG2和SMMC-7721的IC50分别为22.1 mg/L,33.8 mg/L,可诱导HepG2细胞凋亡,而对正常肝细胞LO-2未显示明显的细胞毒性;GC-MS检测分析显示其醚提物主要含12种化学成分.In oglobosum based on fungus’s morphology and its rDNA ITS sequence analyses. The anticancer activity of its metabolites was tested by the MTT method and flow cytometry. Our results indicate that the petroleum ether extract of Chaetomium globosum ZWC5 can induce apoptosis of HepG2 cell with IC50 values of 22. 1 mg/L and 33. 8 mg/L against hepatocellular carcinoma HepG2 and SMMC-7721 cells, respec-tively. It did not show any obvious toxicity against normal liver LO-2 cell. Chemical analysis of its petroleum ether extract by GC-MS displays that the extract contained 12 main chemical constituents.

关 键 词：中华稻蝗 内生真菌 球毛壳霉 抗癌活性 气相色谱-质谱 

分 类 号：Q819[生物学—生物工程]