石榴皮总多酚的微波辅助提取工艺及抗氧化活性研究  

Study on Microwave Assisted Extraction and Antioxidant Activity of Total Polyphenols from Pomegranate Rind

作　　者：黄琦[1,2] 王启越 李颖[1] 叶春林[1,2] 

机构地区：[1]浙江科技学院生物与化学工程学院,浙江杭州310023 [2]浙江省农产品化学与生物加工技术重点实验室,浙江杭州310023

出　　处：《食品研究与开发》2016年第12期46-51,61,共7页Food Research and Development

基　　金：国家级大学生创新训练计划项目(201511057011);浙江省高等学校国内访问学者专业发展项目(2015-58)

摘　　要：利用响应面分析法优化石榴皮总多酚的微波辅助提取工艺,以总多酚得率为指标,通过单因素试验及响应面分析,研究了乙醇体积分数、提取时间、提取功率和液固比4个主要因素对石榴皮总多酚得率的影响。采用BoxBenhnken中心组合设计和响应面分析法,建立了回归方程的预测模型,确定最佳提取条件为乙醇体积分数58%、提取时间98 s、提取功率235 W、液固比49∶1(m L/g)。此条件下总多酚得率为(23.26±0.15)%。试验结果与模型预测值基本相符。用红外光谱对总多酚提取物进行了表征。石榴皮总多酚对DPPH自由基的半清除质量浓度为0.28 mg/m L,具有较强的抗氧化作用。Response surface method was used to optimize the technological conditions of microwave assisted ex-tracting total polyphenols from pomegranate rind. Extraction yield of polyphenols as an indicator, single factor＆amp;nbsp;experiment and response surface method were used to discuss the influence on extraction yield by the ethanol concentration, extraction time, the microwave power and liquid-solid ratio. The regression equation prediction model was developed by Box-Benhnken center combination design and response surface method. The optimal extract conditions were as follows： ethanol concentration 58 %,extracting time 98 s, the microwave power 235 W, liquid-solid ratio 49∶1（mL/g）. Under the condition, the extraction yield of polyphenols was（23.26±0.15）%. The obtained polyphenols was characterized by IR spectra. The IC 50 values of the obtained extracts a-gainst DPPH free radicals was 0.28 mg/mL, suggesting that polyphenols from pomegranate rind had a remark-able antioxidant effect.

关 键 词：石榴皮 总多酚 响应面分析法 微波辅助提取 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]