灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌结构及血流动力学的影响  

Effects of Ganoderma lucidum polysaccharides combined with metformin on myocardial structure and hemodynamics in type 2 diabetic rats

作　　者：乔进[1] 窦志华[1] 吴锋[2] 孟国梁[2] 陈惠[3] 郑惠华[3] 

机构地区：[1]南通市第三人民医院药剂科 [2]南通大学医学院药理学系,江苏南通226001 [3]江苏安惠生物科技有限公司,江苏南通226006

出　　处：《中国药理学通报》2016年第7期1012-1016,共5页Chinese Pharmacological Bulletin

基　　金：科技部十一五国家科技支撑计划项目(No2006DAI06A20-02);江苏省优势学科项目

摘　　要：目的探讨灵芝多糖联合二甲双胍对2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠心肌结构及血流动力学的影响及其机制。方法高脂饮食喂养联合腹腔注射低剂量链脲佐菌素(STZ)30 mg·kg-1建立T2DM大鼠模型。模型动物随机分为模型组、灵芝多糖组(灵芝多糖600 mg·kg^(-1))、二甲双胍组(二甲双胍600 mg·kg^(-1))及联合用药组(灵芝多糖300 mg·kg-1+二甲双胍300 mg·kg-1),另设正常对照组。给药治疗12周末测定大鼠空腹血糖,血流动力学指标(LVSP、LVEDP、dp/dtmax、-dp/dtmax),VG染色法检测大鼠左室心肌胶原容积积分(CVF),免疫组化、蛋白印记法检测心肌组织中基质金属蛋白酶-2(MMP-2)蛋白的表达。结果联合用药组大鼠空腹血糖明显降低;联合用药能明显改善糖尿病大鼠血流动力学指标:降低LVEP,升高LVEDP、dp/dtmax、-dp/dtmax;联合用药组的CVF明显降低;心肌组织MMP-2表达得到明显抑制。结论灵芝多糖联合二甲双胍能明显改善T2DM大鼠血流动力学参数,对糖尿病心肌病变有预防作用,其机制可能与下调MMP-2的表达有关。Aim To discuss the effects and mechanism of Ganoderma lucidum polysaccharides and metformin on myocardial structure and hemodynamics in type 2 diabetic rats.Methods High fat diet combined with intraperitoneal injection of low dose streptozotocin 30 mg· kg -1 was applied to establish rat model of type 2 diabetes mellitus .The diabetic rats were randomly into normal control group ,diabetes group , ganoderma lucid-um polysaccharides group （600 mg· kg -1 ） , metformin group （ 600 mg · kg -1 ） , combination group （ ganoder-ma lucidum polysaccharides 300 mg · kg -1 ＋metform-in 300 mg· kg -1 ） .After 12 weeks′treatment,the lev-els of fasting serum glucose were determined and the hemodynamic parameters （LVSP,LVEDP,dp/dtmax,-dp/dtmax ） were determined.Collagen volume fraction （ CVF ） was detected by Van Gieson . Immunohisto-chemical method and Western blot were used to detect＆nbsp;myocardial tissue MMP-2 protein expression .Results The fasting blood glucose was significantly decreased in the combined treatment group .Combined medication could significantly improve hemodynamic parameters in diabetic rats： reduced LVEP and raised LVEDP , dp/dtmax and -dp/dtmax .CVF was significantly decreased in combination group .The expression of MMP-2 in my-ocardial tissue was significantly inhibited .Conclusions The combination of Ganoderma lucidum polysaccha-ride and metformin can significantly improve the hemo-dynamic parameters in type 2 diabetic rats, and have a preventive effect on diabetic cardiomyopathy . The mechanism may be related to the down regulation of the expression of MMP-2.

关 键 词：灵芝多糖 二甲双胍 糖尿病大鼠 心肌结构 血流动力学 基质金属蛋白酶-2 

分 类 号：R-332[医药卫生] R284.1