乳腺导管内增生性病变中ERα基因启动子甲基化的探讨  

Study on the estrogen receptor- alpha promoter methylation in breast intraductal proliferative lesions

作　　者：毛晓韵[1] 范垂锋[2] 金锋[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,普通外科教研室,乳腺外科,辽宁沈阳110001 [2]中国医科大学基础医学院病理学教研室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2016年第13期2042-2044,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81201886/H1617&81401143/H2501);辽宁省博士科研启动基金项目(编号:20121126)

摘　　要：目的:研究与乳腺浸润性导管癌风险密切相关的导管内增生性病变中ERα基因启动子区甲基化情况。方法:用甲基化特异性PCR研究140例乳腺导管内增生性病变(UDH 40例、ADH 63例、DCIS 37例)的ERα第1、3、4、5启动子甲基化情况。结果:55.0%(22/40)UDH出现ERα基因启动子甲基化,11.1%(7/63)ADH出现ERα基因启动子甲基化,43.2%(16/37)DCIS出现ERα基因启动子甲基化,三者间差异显著(P<0.05)。结论:ERα基因启动子甲基化在乳腺癌的发生发展中是动态变化的,也许其并非是所有分子亚型乳腺癌的始动因素,但这个过程对于细胞的生物学行为改变是非常重要的。Objective：To discuss the promoter methylation of estrogen receptor -alpha in breast intraductal prolif-erative lesions which related to the risk of breast invasive ductal cancer.Methods：The methylation of ERαpromoter was investigated in genomic DNA of 140 breast intraductal proliferative lesions（40 cases of usual ductal hyperplasia, 63 cases of atypical ductal hyperplasia,37 cases of ductal carcinoma in situ）.Results：ERαmethylation was observed in 43.2%（16 /37）ductal carcinoma in situ,which was significantly lower than in usual ductal hyperplasia（55.0%, 22 /40）and was significant higher than in 11.1%（7 /63）atypical ductal hyperplasia（P〈0.05）.Conclusion：The methylation of ERαin breast carcinogenesis was characterized by constant change.Perhaps it was not the initiating factor in breast carcinogenesis of different molecular subtype,but it affected the biological behavior of cells.

关 键 词：乳腺导管内增生性病变 ERΑ 甲基化 

分 类 号：R737.9[医药卫生—肿瘤]